Vibrio vulnificus mutation rate: an in vitro approach

Frontiers in Microbiology

Microbiology

Francisco Jose Roig Molina¹  Jesús Carro Fernández¹  Alejandro Alcaine Otín¹  Carmen Amaro González² 

[1]Computing for Medical and Biological Applications Group, Facultad de Ciencias de la Salud, Universidad San Jorge, Zaragoza, Spain

[2]Departamento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain

[3]Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina BIOTECMED, University of Valencia, Valencia, Spain

关键词: mutation rate;    number of generations;    Vibrio vulnificus;    lineages;    evolution;   

DOI  :  10.3389/fmicb.2023.1223293

 received in 2023-05-15, accepted in 2023-07-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Vibrio vulnificus is a multi-host pathogenic species currently subdivided into five phylogenetic lineages (L) plus one pathovar with the ability to infect fish due to a transmissible virulence plasmid. This plasmid (or a fragment of it) has been transmitted between lineages within the species, contributing to the evolution of V. vulnificus. This study aimed to provide an experimental approximation to the V. vulnificus mutation rate by determining spontaneous mutation rates from bacterial cultures of representants of the different lineages by whole-genome sequencing. To this purpose, synonymous SNP differences, i.e., spontaneous mutation not subjected to the evolutive forces, between initial and final culture after serial growth were evaluated and used for mutation rate calculation.

【 授权许可】

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Copyright © 2023 Roig Molina, Amaro González, Alcaine Otín and Carro Fernández.