In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina

Frontiers in Microbiology

Microbiology

Dolores Rodrigo¹  María Úbeda-Manzanaro¹  Antonio Martínez¹  María Consuelo Pina-Pérez²  Michael Beyrer³ 

[1] Departamento Conservación y Calidad, Instituto de Agroquímica y Tecnología de Alimentos IATA - Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain;Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Valencia, Spain;Food Engineering Laboratory, Institute of Life Technologies, University of Applied Sciences and Arts Western-Switzerland (HES-SO) Valais-Wallis, Sion, Switzerland;Food Engineering Laboratory, Institute of Life Technologies, University of Applied Sciences and Arts Western-Switzerland (HES-SO) Valais-Wallis, Sion, Switzerland;

关键词: Spirulina;    cold atmospheric plasma (CAP);    in vivo;    C. elegans;    antimicrobial activity;    antioxidant activity;   

DOI  :  10.3389/fmicb.2021.781871

 received in 2021-09-23, accepted in 2021-12-24,  发布年份 2022

来源: Frontiers

【 摘 要 】

The present study challenges the in vivo assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of Spirulina powder, using Caenorhabditis elegans as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. C. elegans lifespan and egg laying were used as indicators of antioxidant/antiaging potential of Spirulina (1 mg/mL), when grown with Spirulina CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with Spirulina). According to our results, under both Spirulina supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (p > 0.05) between control and Spirulina CP-treated. Regarding the in vivo assay of the antimicrobial potential of Spirulina against Salmonella enterica serovar Typhimurium (infected worms), no significant differences (p > 0.05) were found between the three exposure scenarios (control [S_0]; Spirulina supplemented media [S_S]; CP-treated Spirulina supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of C. elegans but a reduction in the Spirulina antiaging potential was found. No in vivo modifications in antimicrobial activity seem to be linked to CAPP-processed Spirulina.

【 授权许可】

Unknown   
Copyright © 2022 Pina-Pérez, Úbeda-Manzanaro, Beyrer, Martínez and Rodrigo.