Identification and characterization of circular RNA in the model of autism spectrum disorder from PM2.5 exposure

Frontiers in Genetics

Genetics

Xiaotian Liang¹  Yue Shi²  Bencheng Lin²  Jun Yan²  Kang Li²  Xiaohua Liu²  Lei Tian²  Xiaoqian Xie³  Zhuge Xi³ 

[1] Binzhou Medical University, Yantai, Shandong, China;Tianjin Institute of Environmental and Operational Medicine, Tianjin, China;Tianjin Institute of Environmental and Operational Medicine, Tianjin, China;Binzhou Medical University, Yantai, Shandong, China;

关键词: PM;    circRNA;    autism spectrum disorder (ASD);    transcriptome sequencing;    RNA-seq;   

DOI  :  10.3389/fgene.2023.970465

 received in 2022-06-20, accepted in 2023-04-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

PM2.5 induces a series of effects on neurological disorders, including autism spectrum disorder (ASD), however, the mechanism is not completely clear yet. Circular RNAs (circRNAs) are a class of closed-loop structures that can be stably expressed in vivo. In our experiments, rats exposed to PM2.5 exhibited autism-like phenotypes, such as anxiety, and memory loss. To explore the etiology, we performed transcriptome sequencing and found significant differences in the expression of circRNA. A total of 7770 circRNAs were identified between the control and experimental groups, 18 of which were differentially expressed, we selected ten circRNAs and performed qRT-PCR and Sanger sequencing to validate them. By GO and KEGG enrichment analysis, we found differentially expressed circRNAs that were mainly enriched in processes related to placental development and reproduction. Finally, using bioinformatics, we predicted miRNAs and mRNAs that circ-Mbd5 and circ-Ash1l might regulate and constructed circRNA-miRNA-mRNA networks involving genes associated with ASD, suggesting that circRNAs might regulate the occurrence of ASD.

【 授权许可】

Unknown   
Copyright © 2023 Xie, Li, Liang, Tian, Lin, Yan, Shi, Liu and Xi.