Effects of Cage Position and Light Transmission on Home Cage Activity and Circadian Entrainment in Mice

Frontiers in Neuroscience

Neuroscience

James N. Bussell¹  Manuel Spitschan²  Selma Tir³  Stuart N. Peirson³  Laura C. E. Steel³  Russell G. Foster³  Shu K. E. Tam³ 

[1] Department of Biomedical Services, University of Oxford, Oxford, United Kingdom;Max Planck Institute for Biological Cybernetics, Tübingen, Germany;TUM Department of Sport and Health Sciences (TUM SG), Technical University of Munich, Munich, Germany;Nuffield Department of Clinical Neurosciences, Sir Jules Thorn Sleep and Circadian Neuroscience Institute (SCNi), University of Oxford, Oxford, United Kingdom;

关键词: light;    circadian;    retina;    melanopsin;    cage rack;    individually ventilated cage (IVC);    reproducibility;   

DOI  :  10.3389/fnins.2021.832535

 received in 2021-12-10, accepted in 2021-12-15,  发布年份 2022

来源: Frontiers

【 摘 要 】

Light is known to exert powerful effects on behavior and physiology, including upon the amount and distribution of activity across the day/night cycle. Here we use home cage activity monitoring to measure the effect of differences in home cage light spectrum and intensity on key circadian activity parameters in mice. Due to the relative positioning of any individually ventilated cage (IVC) with regard to the animal facility lighting, notable differences in light intensity occur across the IVC rack. Although all mice were found to be entrained, significant differences in the timing of activity onset and differences in activity levels were found between mice housed in standard versus red filtering cages. Furthermore, by calculating the effective irradiance based upon the known mouse photopigments, a significant relationship between light intensity and key circadian parameters are shown. Perhaps unsurprisingly given the important role of the circadian photopigment melanopsin in circadian entrainment, melanopic illuminance is shown to correlate more strongly with key circadian activity parameters than photopic lux. Collectively, our results suggest that differences in light intensity may reflect an uncharacterized source of variation in laboratory rodent research, with potential consequences for reproducibility. Room design and layout vary within and between facilities, and caging design and lighting location relative to cage position can be highly variable. We suggest that cage position should be factored into experimental design, and wherever possible, experimental lighting conditions should be characterized as a way of accounting for this source of variation.

【 授权许可】

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Copyright © 2022 Steel, Tir, Tam, Bussell, Spitschan, Foster and Peirson.