Bidirectional plasticity of GABAergic tonic inhibition in hippocampal somatostatin- and parvalbumin-containing interneurons

Frontiers in Cellular Neuroscience

Cellular Neuroscience

Marcin Wyroślak¹  Jerzy W. Mozrzymas¹  Grzegorz Dobrzański² 

[1] Department of Biophysics and Neuroscience, Wroclaw Medical University, Wrocław, Poland;Nencki Institute of Experimental Biology, Warsaw, Poland;

关键词: GABA;    tonic inhibition;    hippocampus;    interneurons;    plasticity;    extrasynaptic receptors;   

DOI  :  10.3389/fncel.2023.1193383

 received in 2023-03-24, accepted in 2023-06-05,  发布年份 2023

来源: Frontiers

【 摘 要 】

GABAA receptors present in extrasynaptic areas mediate tonic inhibition in hippocampal neurons regulating the performance of neural networks. In this study, we investigated the effect of NMDA-induced plasticity on tonic inhibition in somatostatin- and parvalbumin-containing interneurons. Using pharmacological methods and transgenic mice (SST-Cre/PV-Cre x Ai14), we induced the plasticity of GABAergic transmission in somatostatin- and parvalbumin-containing interneurons by a brief (3 min) application of NMDA. In the whole-cell patch-clamp configuration, we measured tonic currents enhanced by specific agonists (etomidate or gaboxadol). Furthermore, in both the control and NMDA-treated groups, we examined to what extent these changes depend on the regulation of distinct subtypes of GABAA receptors. Tonic conductance in the somatostatin-containing (SST+) interneurons is enhanced after NMDA application, and the observed effect is associated with an increased content of α5-containing GABAARs. Both fast-spiking and non–fast-spiking parvalbumin-positive (PV+) cells showed a reduction of tonic inhibition after plasticity induction. This effect was accompanied in both PV+ interneuron types by a strongly reduced proportion of δ-subunit-containing GABAARs and a relatively small increase in currents mediated by α5-containing GABAARs. Both somatostatin- and parvalbumin-containing interneurons show cell type-dependent and opposite sign plasticity of tonic inhibition. The underlying mechanisms depend on the cell-specific balance of plastic changes in the contents of α5 and δ subunit-containing GABAARs.

【 授权许可】

Unknown   
Copyright © 2023 Wyroślak, Dobrzański and Mozrzymas.

【 图 表 】

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