GABAAR-mediated tonic inhibition differentially modulates intrinsic excitability of VIP- and SST- expressing interneurons in layers 2/3 of the somatosensory cortex

Frontiers in Cellular Neuroscience

Cellular Neuroscience

Karolina Bogaj¹  Roksana Kaplon¹  Joanna Urban-Ciecko² 

[1] Laboratory of Electrophysiology, Nencki Institute of Experimental Biology, Warsaw, Poland;null;

关键词: barrel cortex;    interneurons;    tonic inhibition;    excitability;    GABA;    somatostatin interneurons;    VIP interneurons;   

DOI  :  10.3389/fncel.2023.1270219

 received in 2023-07-31, accepted in 2023-09-25,  发布年份 2023

来源: Frontiers

【 摘 要 】

Extrasynaptic GABAA receptors (GABAARs) mediating tonic inhibition are thought to play an important role in the regulation of neuronal excitability. However, little is known about a cell type-specific tonic inhibition in molecularly distinctive types of GABAergic interneurons in the mammalian neocortex. Here, we used whole-cell patch-clamp techniques in brain slices prepared from transgenic mice expressing red fluorescent protein (TdTomato) in vasoactive intestinal polypeptide- or somatostatin- positive interneurons (VIP-INs and SST-INs, respectively) to investigate tonic and phasic GABAAR-mediated inhibition as well as effects of GABAA inhibition on intrinsic excitability of these interneurons in layers 2/3 (L2/3) of the somatosensory (barrel) cortex. We found that tonic inhibition was stronger in VIP-INs compared to SST-INs. Contrary to the literature data, tonic inhibition in SST-INs was comparable to pyramidal (Pyr) neurons. Next, tonic inhibition in both interneuron types was dependent on the activity of delta subunit-containing GABAARs. Finally, the GABAAR activity decreased intrinsic excitability of VIP-INs but not SST-INs. Altogether, our data indicate that GABAAR-mediated inhibition modulates neocortical interneurons in a type-specific manner. In contrast to L2/3 VIP-INs, intrinsic excitability of L2/3 SST-INs is immune to the GABAAR-mediated inhibition.

【 授权许可】

Unknown   
Copyright © 2023 Bogaj, Kaplon and Urban-Ciecko.