Granulosa cell-derived extracellular vesicles mitigate the detrimental impact of thermal stress on bovine oocytes and embryos

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Dawit Tesfaye¹  Nico G. Menjivar¹  Ahmed Gad²  Samuel Gebremedhn³  Soham Ghosh⁴ 

[1] Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States;Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States;Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt;Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, United States;Genus Plc, Deforest, WI, United States;Cellular Engineering and Mechanobiology Laboratory (CEML), Department of Mechanical Engineering, Translational Medicine Institute (TMI), Colorado State University, Fort Collins, CO, United States;

关键词: extracellular vesicles;    granulosa cells;    cumulus-oocyte complex;    oocyte maturation;    heat stress;    embryo development;   

DOI  :  10.3389/fcell.2023.1142629

 received in 2023-01-11, accepted in 2023-03-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Climate change-induced global warming results in rises in body temperatures above normal physiological levels (hyperthermia) with negative impacts on reproductive function in dairy and beef animals. Extracellular vesicles (EVs), commonly described as nano-sized, lipid-enclosed complexes, harnessed with a plethora of bioactive cargoes (RNAs, proteins, and lipids), are crucial to regulating processes like folliculogenesis and the initiation of different signaling pathways. The beneficial role of follicular fluid-derived EVs in inducing thermotolerance to oocytes during in vitro maturation (IVM) has been evidenced. Here we aimed to determine the capacity of in vitro cultured granulosa cell-derived EVs (GC-EVs) to modulate bovine oocytes’ thermotolerance to heat stress (HS) during IVM. Moreover, this study tested the hypothesis that EVs released from thermally stressed GCs (S-EVs) shuttle protective messages to provide protection against subsequent HS in bovine oocytes. For this, sub-populations of GC-EVs were generated from GCs subjected to 38.5°C (N-EVs) or 42°C (S-EVs) and supplemented to cumulus-oocyte complexes (COCs) matured in vitro at the normal physiological body temperature of the cow (38.5°C) or HS (41°C) conditions. Results indicate that S-EVs improve the survival of oocytes by reducing ROS accumulation, improving mitochondrial function, and suppressing the expression of stress-associated genes thereby reducing the severity of HS on oocytes. Moreover, our findings indicate a carryover impact from the addition of GC-EVs during oocyte maturation in the development to the blastocyst stage with enhanced viability.

【 授权许可】

Unknown   
Copyright © 2023 Menjivar, Gad, Gebremedhn, Ghosh and Tesfaye.