In vivo evidence for possible up-regulating roles of lysophosphatidic acid around fertilization in rats

The Journal of Veterinary Medical Science

Ryota TERASHIMA¹  Mitsumori KAWAMINAMI²  Shiro KURUSU²  Makoto SUGIYAMA²  Tae TAKEDA²  Miki SHIRASAKA³ 

[1] Laboratory of Veterinary Anatomy, Kitasato University School of Veterinary Medicine, Towada, Aomori 034-8628, Japan;Laboratory of Veterinary Physiology, Kitasato University School of Veterinary Medicine, Towada, Aomori 034-8628, Japan;Present address: Laboratory of Veterinary Surgery, The University of Tokyo Graduate School of Agricultural and Life Sciences, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan

关键词: autotaxin;    cumulus-oocyte complex;    fertilization;    lysophosphatidic acid;    rat;   

DOI  :  10.1292/jvms.17-0354

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Lysophosphatidic acid (LPA) produced by autotaxin (ATX) is recognized as a multi-functional mediator in mammalian reproduction. This study focused on possible effect(s) of LPA on ovulated cumulus-oocyte complexes (COCs) around fertilization in rats in vivo. Immunohistochemistry revealed the cell-type-dependent localization of candidates of synthetic enzymes, ATX and two phospholipases A2 isofroms, and LPA receptors LPA1−4 in ovulated COCs and in oviductal epithelium. The eggs ovulated with a form of COCs became denuded of cumulus cells and underwent fragmentation in the absence of fertilization. In vivo experiments of local administration in non-copulated rats demonstrated that eggs denudation was increased by LPA and decreased by anti-ATX antibody and that fragmentation was inhibited by LPA and stimulated by an ATX chemical inhibitor. Furthermore, LPA administration in adult copulated rats increased the rate of cleaved embryos significantly. Obtained results suggest the presence of LPA synthesis and action system in ovulated COCs within the oviductal ampulla and positive actions of LPA possibly at multiple sites around fertilization in rats.

【 授权许可】

Unknown