Suppressing postcollection lysophosphatidic acid metabolism improves the precision of plasma LPA quantification

Journal of Lipid Research

Nozomu Kono¹  Hirotaka Matsumoto²  Junken Aoki³  Kuniyuki Kano⁴  Yutaka Yatomi⁴  Makoto Kurano⁵ 

[1] AMED-LEAP, Japan Science and Technology Corporation, Kawaguchi, Saitama, Japan;Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-Ku, Sendai, Japan;AMED-LEAP, Japan Science and Technology Corporation, Kawaguchi, Saitama, Japan;Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan;Laboratory of Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-Ku, Sendai, Japan;

关键词: lysophosphatidic acid;    plasma;    clinical specimen;    metabolism;    autotaxin;    LC-MS;   

DOI  :  

来源: DOAJ

【 摘 要 】

Abstract: Lysophosphatidic acid (LPA) is a potent signaling lipid, and state-dependent alterations in plasma LPA make it a promising diagnostic marker for various diseases. However, plasma LPA concentrations vary widely among reports, even under normal conditions. These variations can be attributed, at least in part, to the artificial metabolism of LPA after blood collection. Here, we aimed to develop an optimized plasma preparation method that reflects the concentration of LPA in the circulating blood. The main features of the devised method were suppression of both LPA production and degradation after blood collection by keeping whole blood samples at low temperature followed by the addition of an autotaxin inhibitor to plasma samples. Using this devised method, the LPA level did not change for 30 min after blood collection. Also, human and mouse LPA levels were found to be much lower than those previously reported, ranging from 40 to 50 nM with minimal variation across the individual. Finally, the increased accuracy made it possible to detect circadian rhythms in the levels of certain LPA species in mouse plasma. These results demonstrate the usefulness of the devised plasma preparation method to determine accurate plasma LPA concentrations.

【 授权许可】

Unknown