Genome-wide association analysis reveals the optimal genomic regions for pod size in bean

Frontiers in Plant Science

Plant Science

Xiaohua Wu¹  Jian Wang¹  Guojing Li¹  Wenqi Dong¹  Ying Wang¹  Baogen Wang¹  Yuyan Sun¹  Zhongfu Lu¹  Xinyi Wu²  Jing Yang³  Mao Li⁴  Jian Wu⁴  Junyang Dong⁴ 

[1] Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A & F University, Hangzhou, China;Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A & F University, Hangzhou, China;Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;

关键词: pod size;    GWAS;    KASP marker;    quality;    snap bean;   

DOI  :  10.3389/fpls.2023.1138988

 received in 2023-01-06, accepted in 2023-03-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

The snap bean is the most commonly grown vegetable legume worldwide, and its pod size is both an important yield and appearance quality trait. However, the improvement of pod size in snap beans grown in China has been largely hindered by a lack of information on the specific genes that determine pod size. In this study, we identified 88 snap bean accessions and evaluated their pod size traits. Through a genome-wide association study (GWAS), 57 single nucleotide polymorphisms (SNPs) significantly associated with pod size were detected. Candidate gene analysis showed that cytochrome P450 family genes, WRKY, and MYB transcription factors were the predominant candidate genes for pod development, and eight of these 26 candidate genes showed relatively higher expression patterns in flowers and young pods. A significant pod length (PL) SNP and a single pod weight (SPW) SNP were successfully converted into kompetitive allele-specific polymerase chain reaction (KASP) markers and validated in the panel. These results enhance our understanding of the genetic basis of pod size, and also provide genetic resources for the molecular breeding of pod size in snap beans.

【 授权许可】

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Copyright © 2023 Li, Wu, Wang, Wu, Wang, Wang, Dong, Wu, Lu, Sun, Dong, Yang and Li