Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Xiaowei Liu¹  Ruiqi Xiao²  Peicen Zou²  Yijun Ding³  Jie Wang³  Jinjing Zhang³  Yangfang Li⁴  Yue Du⁵  Yajuan Wang⁵  Ying Li⁵  Jidong Lai⁶  Wenqing Kang⁷ 

[1]Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China

[2]Capital Institute of Pediatrics, Beijing, China

[3]Department of Neonatology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China

[4]Department of Neonatology, Children’s Hospital of Kunming, Kunming, Yunnan, China

[5]Department of Neonatology, Children’s Hospital, Capital Institute of Pediatrics, Beijing, China

[6]Department of Neonatology, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, China

[7]Neonatal Intensive Care Unit, Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou, Henan, China

关键词: neonate;    neonatal infection;    Escherichia coli;    antimicrobial resistance;    MLST;    epidemiology;   

DOI  :  10.3389/fcimb.2023.1183736

 received in 2023-03-10, accepted in 2023-05-08,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundEscherichia coli is one of the most common pathogens causing neonatal infections. Recently, the incidence and drug resistance of E. coli have increased, posing a major threat to neonatal health. The aim of this study was to describe and analyze the antibiotic resistance and multilocus sequence typing (MLST) characteristics of E. coli derived from infants admitted to neonatal intensive care units (NICUs) across China.MethodsIn this study, 370 strains of E. coli from neonates were collected. E. coli isolated from these specimens were subjected to antimicrobial susceptibility testing (by broth microdilution method) and MLST.ResultsThe overall resistance rate was 82.68%, with the highest rate of methicillin/sulfamethoxazole (55.68%) followed by cefotaxime (46.22%). Multiple resistance rate was 36.74%, 132 strains (35.68%) had extended-spectrum β-lactamase (ESBL) phenotype and 5 strains (1.35%) had insensitivity to the tested carbapenem antibiotics. The resistance of E. coli isolated from different pathogenicity and different sites of infections varied, strains derived from sputum were significantly more resistant to β-lactams and tetracyclines. Currently, the prevalence spectrum in NICUs was dominated by ST1193, ST95, ST73, ST69 and ST131 across China. And the multidrug resistance of ST410 was the most severe. ST410 had the highest resistance rate to cefotaxime (86.67%), and its most common multidrug resistance pattern was β-lactams + aminoglycosides + quinolones + tetracyclines + sulfonamides.ConclusionsSubstantial proportions of neonatal E. coli isolates were severely resistant to commonly administered antibiotics. MLST results can suggest the prevalent characteristics of antibiotic resistance in E. coli with different ST types.

【 授权许可】

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Copyright © 2023 Xiao, Li, Liu, Ding, Lai, Li, Kang, Zou, Wang, Du, Zhang and Wang