Metagenomic analysis reveals the virome profiles of Aedes albopictus in Guangzhou, China

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Xiao-Guang Chen¹  Peiwen Liu¹  Tong Liu¹  Ye Xu²  Jiabao Xu² 

[1] Department of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou, China;Department of Pathogen Biology, Institute of Tropical Medicine, School of Public Health, Southern Medical University, Guangzhou, China;School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China;

关键词: Aedes albopictus;    virome;    small RNA sequencing;    mosquito;    virus diversity;   

DOI  :  10.3389/fcimb.2023.1133120

 received in 2022-12-28, accepted in 2023-05-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionAedes albopictus is an aggressive invasive mosquito species widely distributed around the world, and it is also a known vector of arboviruses. Virus metagenomics and RNA interference (RNAi) are important in studying the biology and antiviral defense of Ae. albopictus. However, the virome and potential transmission of plant viruses by Ae. albopictus remain understudied.MethodsMosquito samples of Ae. albopictus were collected from Guangzhou, China, and small RNA sequencing was performed. Raw data were filtered, and virus-associated contigs were generated using VirusDetect. The small RNA profiles were analyzed, and maximum-likelihood phylogenetic trees were constructed.ResultsThe small RNA sequencing of pooled Ae. albopictus revealed the presence of five known viruses, including Wenzhou sobemo-like virus 4, mosquito nodavirus, Aedes flavivirus, Hubei chryso-like virus 1, and Tobacco rattle virus RNA1. Additionally, 21 new viruses that had not been previously reported were identified. The mapping of reads and contig assembly provided insights into the viral diversity and genomic characteristics of these viruses. Field survey confirmed the detection of the identified viruses in Ae. albopictus collected from Guangzhou.DiscussionThe comprehensive analysis of the virus metagenomics of Ae. albopictus in this study sheds light on the diversity and prevalence of viruses in mosquito populations. The presence of known and novel viruses highlights the need for continued surveillance and investigation into their potential impact on public health. The findings also emphasize the importance of understanding the virome and potential transmission of plant viruses by Ae. albopictus.ConclusionThis study provides valuable insights into the virome of Ae. albopictus and its potential role as a vector for both known and novel viruses. Further research is needed to expand the sample size, explore additional viruses, and investigate the implications for public health.

【 授权许可】

Unknown   
Copyright © 2023 Xu, Xu, Liu, Liu and Chen