Metagenomic Sequencing From Mosquitoes in China Reveals a Variety of Insect and Human Viruses

Frontiers in Cellular and Infection Microbiology

Ying Zhang¹  Jingqiang Ren²  Maopeng Wang⁴  Pengpeng Xiao⁴  Huijun Lu⁵  Mingyao Tian⁵  Hao Liu⁶  Yiquan Li⁷  Ningyi Jin⁷  Chenghui Li⁷  Jicheng Han⁷  Lv Xie⁸  Hongning Zhou⁸  Xiaofang Guo⁸ 

[1] College of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, China;Division of Economic Animal Epidemic, Institute of Special Economic Animal and Plant Sciences, Changchun, China;Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China;Institute of Virology, Wenzhou University, Wenzhou, China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China;School of Life Sciences and Engineering, Foshan University, Foshan, China;Yanbian University Medical College, Yanji, China;Yunnan Institute of Parasitic Diseases, Simao, China;

关键词: metagenomic analysis;    mosquito;    virome;    virus detection;    phylogenetic analysis;   

DOI  :  10.3389/fcimb.2018.00364

来源: DOAJ

【 摘 要 】

We collected 8,700 mosquitoes in three sites in China, which belonged to seven species. Their viromes were tested using metagenomic sequencing and bioinformatic analysis. The abundant viral sequences were detected and annotated belonging to more than 50 viral taxonomic families. The results were verified by PCR, followed by phylogenetic analysis. In the present study, we identified partial viral genes of dengue virus (DENV), a novel circovirus (CCV), densovirus (DNV), Japanese encephalitis virus (JEV), and Wuhan mosquito virus (WMV) in mosquitoes. Metagenomic analysis and PCR amplification revealed three DENV sequences, which were as homologous to the NS3 gene of DENV from Singapore isolated in 2005, with at least 91% nucleotide (nt) identity. Seven fragments of JEV encoding structural proteins were identified belonging to genotype I. They all shared high homology with structural protein genes of JEV isolated from Laos in 2009. The production of infectious virus particles of the newly isolated virus YunnanJEV2017-4 increased after passage from the BHK-21 cell line to the Vero cell line. Novel circovirus-related genes were identified and as being related to an unnamed gene of a mosquito circovirus (MCCV) sequence from the USA isolated in 2011, with at least 41% nt identity: this distant relationship suggests that the parent virus might belong to a novel circovirus genus. Additionally, numerous known viruses and some unknown viruses were also detected in mosquitoes from Yunnan province, China, which will be tested for propagation.

【 授权许可】

Unknown