Mouse maternal odontogenic infection with Porphyromonas gingivalis induces cognitive decline in offspring

Frontiers in Pediatrics

Pediatrics

Kazuhiro Tsuga¹  Eri Ishida¹  Hiroshi Oue¹  Hung-Ming Chang²  Ting-Yi Renn²  Yukio Ago³  Ryuji Terayama⁴  Hisako Furusho⁵  Mutsumi Miyauchi⁵  Fumie Shiba⁵ 

[1] Department of Advanced Prosthodontics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Cellular and Molecular Pharmacology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;Department of Maxillofacial Anatomy and Neuroscience, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;Department of Oral and Maxillofacial Pathobiology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;

关键词: maternal exposure;    microglia;    astrocyte;    nervous system disease;    periodontitis;    Porphyromonas gingivalis;   

DOI  :  10.3389/fped.2023.1203894

 received in 2023-04-11, accepted in 2023-07-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionPorphyromonas gingivalis (P. gingivalis), a major periodontal pathogen, causes intrauterine infection/inflammation. Offspring exposed to intrauterine infection/inflammation have an increased risk of neurological disorders, regardless of gestational age. However, the relationship between maternal periodontitis and offspring functional/histological changes in the brain has not yet been elucidated.MethodsIn this study, we used a gestational mouse model to investigate the effects of maternal odontogenic infection of P. gingivalis on offspring behavior and brain tissue.ResultsThe step-through passive avoidance test showed that the latency of the acquisition trial was significantly shorter in the P. gingivalis group (p < 0.05), but no difference in spontaneous motor/exploratory parameters by open-field test. P. gingivalis was diffusely distributed throughout the brain, especially in the hippocampus. In the hippocampus and amygdala, the numbers of neuron cells and cyclic adenosine monophosphate response element binding protein-positive cells were significantly reduced (p < 0.05), whereas the number of ionized calcium binding adapter protein 1-positive microglia was significantly increased (p < 0.05). In the hippocampus, the number of glial fibrillary acidic protein-positive astrocytes was also significantly increased (p < 0.05).DiscussionThe offspring of P. gingivalis-infected mothers have reduced cognitive function. Neurodegeneration/neuroinflammation in the hippocampus and amygdala may be caused by P. gingivalis infection, which is maternally transmitted. The importance of eliminating maternal P. gingivalis-odontogenic infection before or during gestation in maintenance healthy brain function in offspring should be addressed in near future.

【 授权许可】

Unknown   
© 2023 Ishida, Furusho, Renn, Shiba, Chang, Oue, Terayama, Ago, Tsuga and Miyauchi.