Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats

Frontiers in Nutrition

Nutrition

Huijuan Jia¹  Hisanori Kato¹  Xuguang Li¹  Chika Ando¹  Moe Miyoshi¹  Kyohei Furukawa²  Sihui Ma³ 

[1] Health Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;Health Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;Animal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan;Health Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;Faculty of Sport Sciences, Waseda University, Tokorozawa, Japan;

关键词: DNA methylation;    epigenetics;    gene expression;    hypertension;    kidney;    maternal protein restriction;    postnatal nutritional environments;   

DOI  :  10.3389/fnut.2023.1134955

 received in 2022-12-31, accepted in 2023-03-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

Maternal malnutrition hampers the offspring health by manipulating the epigenome. Recent studies indicate that the changes in DNA methylation could be reversed by afterbirth nutrition supplementation. In this study, we used DNA methylation arrays to comprehensively investigate the DNA methylation status of the renal promoter regions and the effects of postnatal protein intake on DNA methylation. We fed stroke-prone spontaneously hypertensive (SHRSP) rat dams a normal diet or a low-protein diet during pregnancy, and their 4-week-old male offspring were fed a normal diet or a high−/low-protein diet for 2 weeks. We found that the methylation status of 2,395 differentially methylated DNA regions was reprogrammed, and 34 genes were reset by different levels of postnatal protein intake in the offspring. Among these genes, Adora2b, Trpc5, Ar, Xrcc2, and Atp1b1 are involved in renal disease and blood pressure regulation. Our findings indicate that postnatal nutritional interventions can potentially reprogram epigenetic changes, providing novel therapeutic and preventive epigenetic targets for salt-sensitive hypertension.

【 授权许可】

Unknown   
Copyright © 2023 Ando, Ma, Miyoshi, Furukawa, Li, Jia and Kato.