Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Frontiers in Sustainable Food Systems

Sustainable Food Systems

Mengmeng Wang¹  Min Yue²  Daodong Pan³  Yangying Sun³  Juan Ni⁴  Xiaoqian Long⁴  Jiangang Ma⁵  Hua Yang⁵  Wen Wang⁵  Biao Tang⁵ 

[1] College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China;Institute of Preventive Veterinary Sciences and Department of Veterinary Medicine, Zhejiang University College of Animal Sciences, Hangzhou, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products and Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products and Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China;

关键词: Enterococcus;    linezolid;    poxtA;    optrA;    Cfr;   

DOI  :  10.3389/fsufs.2023.1179078

 received in 2023-03-03, accepted in 2023-05-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe presence of linezolid-resistant enterococci found in animal-derived food has attracted attention for possible transmission to human-derived enterococci through the food chain. Linezolid-resistant enterococci in farms have been widely reported, but enterococci carrying antimicrobial resistance (AMR) genespoxtA, optrA, or cfr(D) in slaughterhouse environments have not been well addressed.MethodsEnterococcus was isolated from the samples collected from two slaughterhouses in Hangzhou, and the Enterococcus carrying linezolidin-resistant genes was identified by PCR. The minimum inhibitory concentration (MIC) of the Enterococcus carrying linezolidin-resistant genes was determined by microbroth dilution method. Finally, the whole genome of strains carrying two or more linezoline resistance genes was sequenced using the Oxford Nanopore TechnologyResultsHere, 291 enterococci strains were isolated from 309 samples (94.17%). A total of 4 poxtA-positive enterococci and 42 optrA-positive enterococci were identified based on PCR. The antimicrobial susceptibility test showed that the highest rate of florfenicol resistance was 97.82% and the rate of multidrug resistance (MDR) was 95.65%. Two strains carried multiple linezolid resistance genes, among which Enterococcus casseliflavus CQFYY22-063 cocarryingoptrA, poxtA, and cfr(D) was isolated from the duck cecum, and Enterococcus faecium CQFYH22-006 cocarryingoptrA and poxtA was isolated from slaughterhouse sewage for the first time. Furthermore, Oxford Nanopore Technology revealed that the optrA gene of strain CQFYY22-063 was located on the Inc18-type plasmid pFYY063-optrA-70K, and thepoxtA and cfr(D) genes were located on the Inc18-type plasmid pFYY063- poxtA-12K. Meanwhile, the poxtA gene of strain CQFYH22-006 was located on the Rep3-type plasmid pFYH006-poxtA-25K, and the optrA gene was located on the chromosome.DiscussionTogether, linezolid resistance in slaughterhouses deserves extensive attention, indicating the need to strengthen the monitoring of different links in the food production chain within the One Health concept.

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Copyright © 2023 Ni, Long, Wang, Ma, Sun, Wang, Yue, Yang, Pan and Tang.