Virulence-related factors and antimicrobial resistance in Proteus mirabilis isolated from domestic and stray dogs

Frontiers in Microbiology

Microbiology

Haifeng Liu¹  Zhiyou Dong¹  Ziyao Zhou¹  Hualin Fu¹  Guangneng Peng¹  Shanyu Chen¹  Zhihua Ren¹  Xiaoping Ma¹  Qianlan Li¹  Yanchun Hu¹  Zhijun Zhong¹  Lijuan Liu¹  Gang Shu¹  Xianmeng Qiu²  Shengquan Ai²  Mengyao Dong³ 

[1] Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China;New Ruipeng Pet Healthcare Group, Chengdu, China;State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China;

关键词: Proteus mirabilis;    virulence-related factors;    antimicrobial resistance;    antibiotic resistance genes;    virulence-associated genes;    domestic and stray dogs;   

DOI  :  10.3389/fmicb.2023.1141418

 received in 2023-01-10, accepted in 2023-04-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionProteus mirabilis is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. Proteus mirabilis is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on P. mirabilis isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs).MethodIn this study, we isolated 75 strains of P. mirabilis from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates.ResultsOur findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among P. mirabilis isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry ureC, FliL, ireA, zapA, ptA, hpmA, hpmB, pmfA, rsbA, mrpA, and ucaA with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry aac(6′)-Ib, qnrD, floR, blaCTX-M, blaCTX-M-2, blaOXA-1, blaTEM, tetA, tetB and tetM with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: blaTEM, blaCTX-M, and blaCTX-M-2. This study revealed that P. mirabilis strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs.DiscussionGiven the increasing antimicrobial resistance of P. mirabilis, veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health.

【 授权许可】

Unknown   
Copyright © 2023 Liu, Dong, Ai, Chen, Dong, Li, Zhou, Liu, Zhong, Ma, Hu, Ren, Fu, Shu, Qiu and Peng.