Targeted genome editing in polyploids: lessons from Brassica

Frontiers in Plant Science

Plant Science

Muhammad Aamer Mehmood¹  Qamar U. Zaman²  Rafaqat Ali Gill³  Weijun Zhou⁴  Rana Muhammad Atif⁵  Samia Fatima⁶  Mehboob-ur Rahman⁶  Niaz Ahmad⁶ 

[1] Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan;Hainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, Sanya, China;College of Tropical Crops, Hainan University, Haikou, China;Key Laboratory for Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China;Ministry of Agriculture and Rural Affairs Key Lab of Spectroscopy Sensing, Institute of Crop Science, Zhejiang University, Hangzhou, China;National Center of Genome Editing, Center of Advanced Studies, Agriculture and Food Security, University of Agriculture, Faisalabad, Pakistan;Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad, Pakistan;National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, Pakistan;

关键词: genome editing;    CRISPR;    Brassica;    polyploid crops;    crop improvement;   

DOI  :  10.3389/fpls.2023.1152468

 received in 2023-01-27, accepted in 2023-04-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

CRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the genome for various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, and increasing yield and quality. However, its utilization is limited to model crops for which well-annotated genome sequences are available. Many crops of dietary and economic importance, such as wheat, cotton, rapeseed-mustard, and potato, are polyploids with complex genomes. Therefore, progress in these crops has been hampered due to genome complexity. Excellent work has been conducted on some species of Brassica for its improvement through genome editing. Although excellent work has been conducted on some species of Brassica for genome improvement through editing, work on polyploid crops, including U’s triangle species, holds numerous implications for improving other polyploid crops. In this review, we summarize key examples from genome editing work done on Brassica and discuss important considerations for deploying CRISPR-mediated genome editing more efficiently in other polyploid crops for improvement.

【 授权许可】

Unknown   
Copyright © 2023 Ahmad, Fatima, Mehmood, Zaman, Atif, Zhou, Rahman and Gill