Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections

Viruses

Yuan-Chuan Chen¹  Fenyong Liu¹  Jingxue Sheng²  Phong Trang² 

[1] College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China;Program in Comparative Biochemistry, University of California, Berkeley, CA 94720, USA;

关键词: CRISPR;    clinical application;    herpesvirus;    latent infection;    genome editing;    HSV (Herpes Simplex Virus);    EBV (Epstein–Barr Virus);    CMV (cytomegalovirus);    KSHV (Kaposi’s Sarcoma-Associated Herpesvirus);   

DOI  :  10.3390/v10060291

来源: DOAJ

【 摘 要 】

The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein–Barr virus, cytomegalovirus, and Kaposi’s sarcoma-associated herpesvirus. CRISPR/Cas9-directed mutagenesis can introduce similar types of mutations to the viral genome as can bacterial artificial chromosome recombination engineering, which maintains and reconstitutes the viral genome successfully. The cleavage mediated by CRISPR/Cas9 enables the manipulation of disease-associated viral strains with unprecedented efficiency and precision. Additionally, current therapies for herpesvirus productive and latent infections are limited in efficacy and cannot eradicate viruses. CRISPR/Cas9 is potentially adapted for antiviral treatment by specifically targeting viral genomes during latent infections. This review, which focuses on recently published progress, suggests that the CRISPR/Cas9 system is not only a useful tool for basic virology research, but also a promising strategy for the control and prevention of herpesvirus latent infections.

【 授权许可】

Unknown