Investigation of Pseudorabies Virus Latency in Nervous Tissues of Seropositive Pigs Exposed to Field Strain

Journal of Veterinary Medical Science

John-Hwa LEE⁴  Joon-Seok CHAE¹  Abi George ALEYAS⁵  Se-Yeoun CHA⁵  Hyun-A. YOON⁵  Hee-Jong SONG³  Hyung-Kwan JANG²  eong-Gon CHO⁵  Seong-Kug EO⁵ 

[1] 3Laboratory of Clinical Pathology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University;Laboratory of Avian Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University;Laboratory of Infectous Diseases, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University;Laboratory of Public Hygiene, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University;Laboratory of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University

关键词: latent infection;    nested PCR;    pseudorabies virus;    real-time PCR;   

DOI  :  10.1292/jvms.68.143

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Cited-By(4)The prevalence and quantity of latent pseudorabies virus (PrV) in nervous tissues of pigs exposed to field strain in Korea was investigated by nested and real-time PCR. Nervous tissues including trigeminal ganglion (TG), olfactory bulb (OB), and brain stem (BS) were collected from 94 seropositive pigs. PrV latent infection in nervous tissues was initially investigated by nested PCR targeting three glycoprotein genes (gB, gE, and gG). Based on the obtained result, latent infection was detected in 95.7% of screened animals. Furthermore, it was revealed that the examined tissues harbored different copy numbers of latent PrV genome ranging from <102.0 to 107.1 copies per microgram of genomic DNA in real-time PCR analysis. These results show that under normal conditions, levels of latent PrV in the nervous tissues of pigs can vary across a wide range. Therefore, the data presented here provides information regarding control of the endemic state of PrV in Korea.

【 授权许可】

Unknown