Rickettsia association with two Macrolophus (Heteroptera: Miridae) species: A comparative study of phylogenies and within-host localization patterns

Frontiers in Microbiology

Microbiology

Netta Mozes-Daube¹  Einat Zchori-Fein¹  Yehuda Izraeli²  Moshe Coll³  Maria Dally⁴  Eduard Belausov⁵ 

[1] Department of Entomology, Newe-Ya’ar Research Center, ARO, Ramat-Yishay, Israel;Department of Entomology, Newe-Ya’ar Research Center, ARO, Ramat-Yishay, Israel;Department of Evolution and Environmental Biology, University of Haifa, Haifa, Israel;Department of Entomology, RH Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel;Department of Entomology, RH Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel;Department of Entomology, Newe-Ya’ar Research Center, ARO, Ramat-Yishay, Israel;The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeZion, Israel;

关键词: FISH;    Macrolophus melanotoma;    Macrolophus pygmaeus;    omnivory;    Rickettsia bellii;    Rickettsia limoniae;   

DOI  :  10.3389/fmicb.2022.1107153

 received in 2022-11-24, accepted in 2022-12-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Many arthropods host bacterial symbionts, some of which are known to influence host nutrition and diet breadth. Omnivorous bugs of the genus Macrolophus (Heteroptera: Miridae) are mainly predatory, but may also feed on plants. The species M. pygmaeus and M. melanotoma (=M. caliginosus) are key natural enemies of various economically important agricultural pests, and are known to harbor two Rickettsia species, R. bellii and R. limoniae. To test for possible involvement of symbiotic bacteria in the nutritional ecology of these biocontrol agents, the abundance, phylogeny, and distribution patterns of the two Rickettsia species in M. pygmaeus and M. melanotoma were studied. Both of the Rickettsia species were found in 100 and 84% of all tested individuals of M. pygmaeus and M. melanotoma, respectively. Phylogenetic analysis showed that a co-evolutionary process between Macrolophus species and their Rickettsia is infrequent. Localization of R. bellii and R. limoniae has been detected in both female and male of M. pygmaeus and M. melanotoma. FISH analysis of female gonads revealed the presence of both Rickettsia species in the germarium of both bug species. Each of the two Rickettsia species displayed a unique distribution pattern along the digestive system of the bugs, mostly occupying separate epithelial cells, unknown caeca-like organs, the Malpighian tubules and the salivary glands. This pattern differed between the two Macrolophus species: in M. pygmaeus, R. limoniae was distributed more broadly along the host digestive system and R. bellii was located primarily in the foregut and midgut. In contrast, in M. melanotoma, R. bellii was more broadly distributed along the digestive system than the clustered R. limoniae. Taken together, these results suggest that Rickettsia may have a role in the nutritional ecology of their plant-and prey-consuming hosts.

【 授权许可】

Unknown   
Copyright © 2023 Dally, Izraeli, Belausov, Mozes-Daube, Coll and Zchori-Fein.