Shortwave infrared fluorescence imaging of peripheral organs in awake and freely moving mice

Frontiers in Neuroscience

Neuroscience

Ellen M. Sletten¹  Joycelyn Yiu²  Ilaria Balba²  Emily D. Cosco³  Thomas S. Bischof⁴  Bernardo A. Arús⁴  Oliver T. Bruns⁴ 

[1] Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, United States;Helmholtz Pioneer Campus, Helmholtz Zentrum München, Neuherberg, Germany;Helmholtz Pioneer Campus, Helmholtz Zentrum München, Neuherberg, Germany;Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, United States;Helmholtz Pioneer Campus, Helmholtz Zentrum München, Neuherberg, Germany;German Cancer Research Center (DKFZ), Heidelberg, Germany;National Center for Tumor Diseases (NCT/UCC), Dresden, Germany;Medizinische Fakultät and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany;Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany;

关键词: optical imaging;    fluorescence;    in vivo;    awake imaging;    fluorescent dyes;    near infrared (NIR)-II;    organic fluorophores;    polymethine dyes;   

DOI  :  10.3389/fnins.2023.1135494

 received in 2022-12-31, accepted in 2023-04-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Extracting biological information from awake and unrestrained mice is imperative to in vivo basic and pre-clinical research. Accordingly, imaging methods which preclude invasiveness, anesthesia, and/or physical restraint enable more physiologically relevant biological data extraction by eliminating these extrinsic confounders. In this article, we discuss the recent development of shortwave infrared (SWIR) fluorescent imaging to visualize peripheral organs in freely-behaving mice, as well as propose potential applications of this imaging modality in the neurosciences.

【 授权许可】

Unknown   
Copyright © 2023 Arús, Cosco, Yiu, Balba, Bischof, Sletten and Bruns.