Effect of quercetin on steroidogenesis and folliculogenesis in ovary of mice with experimentally-induced polycystic ovarian syndrome

Frontiers in Endocrinology

Endocrinology

Wajid Mohammad Sheikh¹  Showkeen Muzamil Bashir¹  Ebtesam A. Al-Suhaimi²  Meneerah A. Al-Jafary²  Gulzar Ahmed Rather³  Manzoor Ahmad Mir⁴  Mohd Ashraf Ganie⁵  Abdalelgadir Musa Homeida⁶  Mohammad Azam Ansari⁷  Mohammad H. Al-Qhtani⁸  Majid Shafi⁹  Mohd Zahoor Ul Haq Shah^1,10  Vinoy Kumar Shrivastva^1,10 

[1] Biochemistry & Molecular Biology Lab, Division of Veterinary Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India;Biology Department, College of Science and Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;Department of Biomedical Engineering Sathyabama Institute of Science & Technology, Chennai, Tamil Nadu, India;Department of Bioresources, University of Kashmir, Srinagar, Jammu and Kashmir, India;Department of Endocrinology and Metabolism, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, India;Department of Environmental Health Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;Department of Epidemic Disease Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;Department of Paediatrics, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Jammu and Kashmir, India;Laboratory of Endocrinology, Department of Bioscience Barkatullah University Bhopal, Madhya Pradesh, India;

关键词: PCOS (polycystic ovarian syndrome);    steroidogenesis;    folliculogenesis;    quercetin;    VEGF;   

DOI  :  10.3389/fendo.2023.1153289

 received in 2023-01-30, accepted in 2023-04-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionPolycystic Ovary syndrome (PCOS) affects the health of many women around theworld. Apart from fundamental metabolic problems connected to PCOS, focus of our study is on the role of quercetin on genes relevant to steroidogenesis and folliculogenesis. MethodsEighteen mature parkes strain mice (4-5 weeks old) weighing18–21 g were randomly divided into three groups of six each as follows: Group I serves as the control and was given water and a regular chow diet ad lib for 66 days; group II was given oral gavage administration of letrozole (LETZ) (6 mg/kgbw) for 21 days to induce PCOS and was left untreated for 45 days; For three weeks, Group III received oral gavage dose of LETZ (6 mg/kg), after which it received Quercetin (QUER) (125 mg/kg bw orally daily) for 45 days.ResultsIn our study we observed that mice with PCOS had irregular estrous cycle with increased LH/FSH ratio, decreased estrogen level and decline in expression of Kitl, Bmp1, Cyp11a1, Cyp19a1, Ar, lhr, Fshr and Esr1 in ovary. Moreover, we observed increase in the expression of CYP17a1, as well as increase in cholesterol, triglycerides, testosterone, vascular endothelial growth factor VEGF and insulin levels. All these changes were reversed after the administration of quercetin in PCOS mice.DiscussionQuercetin treatment reversed the molecular, functional and morphological abnormalities brought on due to letrozole in pathological and physiological setting, particularly the issues of reproduction connected to PCOS. Quercetin doesn’t act locally only but it acts systematically as it works on Pituitary (LH/FSH)- Ovary (gonad hormones) axis. the Side effects of Quercetin have to be targeted in future researches. Quercetin may act as a promising candidate for medical management of human PCOS.

【 授权许可】

Unknown   
Copyright © 2023 Shah, Shrivastva, Mir, Sheikh, Ganie, Rather, Shafi, Bashir, Ansari, Al-Jafary, Al-Qhtani, Homeida and Al-Suhaimi