Alterations in the gut microbiome and its metabolites are associated with the immune response to mucosal immunization with Lactiplantibacillus plantarum-displaying recombinant SARS-CoV-2 spike epitopes in mice

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Robie Vasquez¹  In-Chan Hwang¹  Ji Hoon Song¹  Dae-Kyung Kang¹  Lars Engstrand²  Valerie Diane Valeriano² 

[1] Department of Animal Biotechnology, Dankook University, Cheonan, Republic of Korea;Department of Microbiology, Tumor and Cell Biology, Centre for Translational Microbiome Research (CTMR), Karolinska Institutet, Stockholm, Sweden;

关键词: gut microbiome;    mucosal vaccine;    SARS-CoV-2;    COVID-19;    lactic acid bacteria;    butyrate;   

DOI  :  10.3389/fcimb.2023.1242681

 received in 2023-06-19, accepted in 2023-08-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

Lactic acid bacteria (LAB) expressing foreign antigens have great potential as mucosal vaccines. Our previous study reported that recombinant Lactiplantibacillus plantarum SK156 displaying SARS-CoV-2 spike S1 epitopes elicited humoral and cell-mediated immune responses in mice. Here, we further examined the effect of the LAB-based mucosal vaccine on gut microbiome composition and function, and gut microbiota-derived metabolites. Forty-nine (49) female BALB/c mice were orally administered L. plantarum SK156-displaying SARS-CoV-2 spike S1 epitopes thrice (at 14-day intervals). Mucosal immunization considerably altered the gut microbiome of mice by enriching the abundance of beneficial gut bacteria, such as Muribaculaceae, Mucispirillum, Ruminococcaceae, Alistipes, Roseburia, and Clostridia vadinBB60. Moreover, the predicted function of the gut microbiome showed increased metabolic pathways for amino acids, energy, carbohydrates, cofactors, and vitamins. The fecal concentration of short-chain fatty acids, especially butyrate, was also altered by mucosal immunization. Notably, alterations in gut microbiome composition, function, and butyrate levels were positively associated with the immune response to the vaccine. Our results suggest that the gut microbiome and its metabolites may have influenced the immunogenicity of the LAB-based SARS-CoV-2 vaccine.

【 授权许可】

Unknown   
Copyright © 2023 Hwang, Vasquez, Song, Engstrand, Valeriano and Kang