Transcriptomics reveal the genetic coordination of early defense to Armillaria root rot (ARR) in Prunus spp

Frontiers in Plant Science

Plant Science

Jeffrey Adelberg¹  Ksenija Gasic¹  Jacqueline Naylor-Adelberg¹  Gregory Reighard¹  Christopher A. Saski¹  Lichun Cai¹  Alejandro Calle¹  Zhigang Li¹  Guido Schnabel¹ 

[1] Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States;

关键词: Armillaria;    transcriptomics;    Prunus;    rootstock;    fungi;   

DOI  :  10.3389/fpls.2023.1181153

 received in 2023-03-07, accepted in 2023-05-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Armillaria root rot (ARR) poses a significant threat to the long-term productivity of stone-fruit and nut crops in the predominant production area of the United States. To mitigate this issue, the development of ARR-resistant and horticulturally-acceptable rootstocks is a crucial step towards the maintenance of production sustainability. To date, genetic resistance to ARR has been found in exotic plum germplasm and a peach/plum hybrid rootstock, ’MP-29‘. However, the widely-used peach rootstock Guardian® is susceptible to the pathogen. To understand the molecular defense mechanisms involved in ARR resistance in Prunus rootstocks, transcriptomic analyses of one susceptible and two resistant Prunus spp. were performed using two causal agents of ARR, including Armillaria mellea and Desarmillaria tabescens. The results of in vitro co-culture experiments revealed that the two resistant genotypes showed different temporal response dynamics and fungus-specific responses, as seen in the genetic response. Gene expression analysis over time indicated an enrichment of defense-related ontologies, including glucosyltransferase activity, monooxygenase activity, glutathione transferase activity, and peroxidase activity. Differential gene expression and co-expression network analysis highlighted key hub genes involved in the sensing and enzymatic degradation of chitin, GSTs, oxidoreductases, transcription factors, and biochemical pathways likely involved in Armillaria resistance. These data provide valuable resources for the improvement of ARR resistance in Prunus rootstocks through breeding.

【 授权许可】

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Copyright © 2023 Cai, Adelberg, Naylor-Adelberg, Schnabel, Calle, Li, Reighard, Gasic and Saski