Major transcriptomic differences are induced by warmer temperature conditions experienced during asexual and sexual reproduction in Fragaria vesca ecotypes

Frontiers in Plant Science

Plant Science

Timo Hytönen¹  Marcos Viejo²  Torstein Tengs³  Carl Gunnar Fossdal³  Igor Yakovlev³  Paal Krokene³  Yupeng Zhang⁴  Paul E. Grini⁵ 

[1] Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland;Department of Functional Biology, University of Santiago de Compostela, Santiago de Compostela, Spain;Department of Molecular Plant Biology, Norwegian Institute of Bioeconomy Research, Ås, Norway;Department of Molecular Plant Biology, Norwegian Institute of Bioeconomy Research, Ås, Norway;EVOGENE, Department of Biosciences, University of Oslo, Oslo, Norway;EVOGENE, Department of Biosciences, University of Oslo, Oslo, Norway;

关键词: Fragaria vesca;    asexual (clonal) reproduction;    sexual reproduction;    epigenetics;    transcriptome (RNA-seq);   

DOI  :  10.3389/fpls.2023.1213311

 received in 2023-04-27, accepted in 2023-06-29,  发布年份 2023

来源: Frontiers

【 摘 要 】

A major challenge for plants in a rapidly changing climate is to adapt to rising temperatures. Some plants adapt to temperature conditions by generating an epigenetic memory that can be transmitted both meiotically and mitotically. Such epigenetic memories may increase phenotypic variation to global warming and provide time for adaptation to occur through classical genetic selection. The goal of this study was to understand how warmer temperature conditions experienced during sexual and asexual reproduction affect the transcriptomes of different strawberry (Fragaria vesca) ecotypes. We let four European F. vesca ecotypes reproduce at two contrasting temperatures (18 and 28°C), either asexually through stolon formation for several generations, or sexually by seeds (achenes). We then analyzed the transcriptome of unfolding leaves, with emphasis on differential expression of genes belonging to the epigenetic machinery. For asexually reproduced plants we found a general transcriptomic response to temperature conditions but for sexually reproduced plants we found less significant responses. We predicted several splicing isoforms for important genes (e.g. a SOC1, LHY, and SVP homolog), and found significantly more differentially presented splicing event variants following asexual vs. sexual reproduction. This difference could be due to the stochastic character of recombination during meiosis or to differential creation or erasure of epigenetic marks during embryogenesis and seed development. Strikingly, very few differentially expressed genes were shared between ecotypes, perhaps because ecotypes differ greatly both genetically and epigenetically. Genes related to the epigenetic machinery were predominantly upregulated at 28°C during asexual reproduction but downregulated after sexual reproduction, indicating that temperature-induced change affects the epigenetic machinery differently during the two types of reproduction.

【 授权许可】

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Copyright © 2023 Zhang, Viejo, Yakovlev, Tengs, Krokene, Hytönen, Grini and Fossdal