Identification and Analysis of Mitogen-Activated Protein Kinase (MAPK) Cascades in Fragaria vesca

International Journal of Molecular Sciences

Yu Xing¹  Yuanfang Han¹  Ling Qin¹  Qing Zhang¹  Heying Zhou²  Suyue Ren² 

[1] Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry and Fruit Trees, Beijing University of Agriculture, Beijing 102206, China;These authors contributed equally to this work.;

关键词: Fragaria vesca;    MAP kinase cascades;    phylogenetic relationships;    abiotic stresses;    gene expression;   

DOI  :  10.3390/ijms18081766

来源: DOAJ

【 摘 要 】

Mitogen-activated protein kinase (MAPK) cascades are highly conserved signaling modules in eukaryotes, including yeasts, plants and animals. MAPK cascades are responsible for protein phosphorylation during signal transduction events, and typically consist of three protein kinases: MAPK, MAPK kinase, and MAPK kinase kinase. In this current study, we identified a total of 12 FvMAPK, 7 FvMAPKK, 73 FvMAPKKK, and one FvMAPKKKK genes in the recently published Fragaria vesca genome sequence. This work reported the classification, annotation and phylogenetic evaluation of these genes and an assessment of conserved motifs and the expression profiling of members of the gene family were also analyzed here. The expression profiles of the MAPK and MAPKK genes in different organs and fruit developmental stages were further investigated using quantitative real-time reverse transcription PCR (qRT-PCR). Finally, the MAPK and MAPKK expression patterns in response to hormone and abiotic stresses (salt, drought, and high and low temperature) were investigated in fruit and leaves of F. vesca. The results provide a platform for further characterization of the physiological and biochemical functions of MAPK cascades in strawberry.

【 授权许可】

Unknown