Immunogenicity and protective efficacy of nanoparticle formulations of L-SseB against Salmonella infection

Frontiers in Immunology

Immunology

Robert K. Ernst¹  David J. Varisco¹  Siva S. K. Ratnakaram²  Debaki R. Howlader²  Wendy L. Picking²  Ti Lu²  Sayan Das²  Zackary K. Dietz²  William D. Picking²  Gang Hu²  Sean K. Whittier²  Simran Sharma³ 

[1] Department of Microbial Pathogenesis, University of Maryland, Baltimore, MD, United States;Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, United States;Department of Veterinary Pathobiology and Bond Life Science Center, University of Missouri, Columbia, MO, United States;

关键词: Salmonella;    T3SS;    vaccine;    formulation;    typhoid;    IL-17;   

DOI  :  10.3389/fimmu.2023.1208848

 received in 2023-04-19, accepted in 2023-06-13,  发布年份 2023

来源: Frontiers

【 摘 要 】

Salmonella enterica, a Gram-negative pathogen, has over 2500 serovars that infect a wide range of hosts. In humans, S. enterica causes typhoid or gastroenteritis and is a major public health concern. In this study, SseB (the tip protein of the Salmonella pathogenicity island 2 type III secretion system) was fused with the LTA1 subunit of labile-toxin from enterotoxigenic E. coli to make the self-adjuvanting antigen L-SseB. Two unique nanoparticle formulations were developed to allow multimeric presentation of L-SseB. Mice were vaccinated with these formulations and protective efficacy determined via challenging the mice with S. enterica serovars. The polysaccharide (chitosan) formulation was found to elicit better protection when compared to the squalene nanoemulsion. When the polysaccharide formulation was used to vaccinate rabbits, protection from S. enterica challenge was elicited. In summary, L-SseB in a particulate polysaccharide formulation appears to be an attractive candidate vaccine capable of broad protection against S. enterica.

【 授权许可】

Unknown   
Copyright © 2023 Das, Howlader, Lu, Whittier, Hu, Sharma, Dietz, Ratnakaram, Varisco, Ernst, Picking and Picking