Genetic analysis of heat tolerance in hot pepper: insights from comprehensive phenotyping and QTL mapping

Frontiers in Plant Science

Plant Science

Ruchi Gupta¹  Bhupinder Singh²  Hari Krishna³  Renu Pandey⁴  Manisha Mangal⁵  Arpita Srivastava⁵  Aruna TS⁵  Bhoopal Singh Tomar⁵  Tusar Kanti Behera⁶  Pradeep Kumar Jain⁷ 

[1] Department of Computer Sciences, Jamia Milia Islamia, New Delhi, India;Division of Environment Science, Indian Agricultural Research Institute, Indian Council of Agricultural Research (ICAR), New Delhi, India;Division of Genetics, Indian Agricultural Research Institute, Indian Council of Agricultural Research (ICAR), New Delhi, India;Division of Plant Physiology, Indian Agricultural Research Institute, Indian Council of Agricultural Research (ICAR), New Delhi, India;Division of Vegetable Science, Indian Agricultural Research Institute, Indian Council of Agricultural Research (ICAR), New Delhi, India;Indian Council of Agricultural Research-Indian Institute of Vegetable Research (IIVR), Indian Council of Agricultural Research (ICAR), Varanasi, India;National Institute of Plant Biotechnology, Indian Council of Agricultural Research (ICAR), New Delhi, India;

关键词: Capsicum annuum;    heat tolerance;    QTL mapping;    additive effect;    dominance effect;    phenotyping;    genotyping;   

DOI  :  10.3389/fpls.2023.1232800

 received in 2023-06-01, accepted in 2023-07-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

High temperatures present a formidable challenge to the cultivation of hot pepper, profoundly impacting not only vegetative growth but also leading to flower and fruit abscission, thereby causing a significant reduction in yield. To unravel the intricate genetic mechanisms governing heat tolerance in hot pepper, an F2 population was developed through the crossing of two distinct genotypes exhibiting contrasting heat tolerance characteristics: DLS-161-1 (heat tolerant) and DChBL-240 (heat susceptible). The F2 population, along with the parental lines, was subjected to comprehensive phenotyping encompassing diverse morphological, physiological, and biochemical heat-related traits under high temperature conditions (with maximum temperature ranging from 31 to 46.5°C and minimum temperature from 15.4 to 30.5°C). Leveraging the Illumina Nova Seq-6000 platform, Double digest restriction-site associated DNA sequencing (ddRAD-seq) was employed to generate 67.215 Gb data, with subsequent alignment of 218.93 million processed reads against the reference genome of Capsicum annuum. Subsequent variant calling and ordering resulted in 5806 polymorphic SNP markers grouped into 12 LGs. Further QTL analysis identified 64 QTLs with LOD values ranging from 2.517 to 11.170 and explained phenotypic variance ranging from 4.05 to 19.39%. Among them, 21 QTLs, explaining more than 10% phenotypic variance, were identified as major QTLs controlling 9 morphological, 3 physiological, and 2 biochemical traits. Interestingly, several QTLs governing distinct parameters were found to be colocalized, suggesting either a profound correlation between the QTLs regulating these traits or their significant genomic proximity. In addition to the QTLs, we also identified 368380 SSR loci within the identified QTL regions, dinucleotides being the most abundant type (211,381). These findings provide valuable insights into the genetics of heat tolerance in hot peppers. The identified QTLs and SSR markers offer opportunities to develop heat-tolerant varieties, ensuring better crop performance under high-temperature conditions.

【 授权许可】

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Copyright © 2023 TS, Srivastava, Tomar, Behera, Krishna, Jain, Pandey, Singh, Gupta and Mangal