Genes and Environment | |
Additive Mutagenic Effects of DNA Damages Induced by Multiple Mutagens at Virtually Non-mutagenic Dose Level of Each | |
Toshihiro Ohta1  | |
[1] School of Life Sciences, Tokyo University of Pharmacy and Life Sciences | |
关键词: threshold; nucleotide excision repair; combined mutagenicity; additive effect; Salmonella typhimurium; | |
DOI : 10.3123/jemsge.29.141 | |
学科分类:分子生物学,细胞生物学和基因 | |
来源: Japanese Environmental Mutagen Society / Nihon Kankyo Hen igen Gakkai | |
【 摘 要 】
References(23)DNA damages provoked by the six mutagens (furylframide, MX, 4-nitroquinoline N-oxide, sodium azide, 1-nitropyrene, and captan) used in the present study have been known to subject to the nucleotide excision repair system. Therefore, they induced base-substitution mutations much more efficiently in Salmonella typhimurium TA100 (hisG46, rfa, uvrB/pKM101), a strain deficient in nucleotide excision repair, than in TA1975P (hisG46, rfa/pKM101). Doses below undetectable level in the reverse mutation assay were selected as those that were apparently mutagenic to the repair-deficient strain TA100 but not to the repair-proficient strain TA1975P in this study. Then, the six mutagens were mixed at virtually non-mutagenic dose level of each and a possible combined mutagenic effect was investigated. A significant and reproducible increase in the number of revertants in TA1975P was observed with combined mutagens. The results suggest that DNA adduct formation at virtually non-mutagenic dose level was additive, with the total amount reflecting the mutagenicity.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912080716069ZK.pdf | 222KB | download |