Babesia microti alleviates disease manifestations caused by Plasmodium berghei ANKA in murine co-infection model of complicated malaria

Frontiers in Cellular and Infection Microbiology

Cellular and Infection Microbiology

Hanadi B. Baghdadi¹  Tomoyo Taniguchi²  Mohamed Abdo Rizk³  Daisuke Kondoh⁴  Hang Li⁵  Shengwei Ji⁵  Ma Zhuowei⁵  Thom Do⁵  Xuenan Xuan⁵  Ma Yihong⁵  Noboru Inoue⁵  Moaz M. Amer⁵  Eloiza May Galon⁶  Shimaa Abd El-Salam El-Sayed⁷  Iqra Zafar⁸  Jinlin Zhou⁹ 

[1]Biology Department, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia

[2]Basic and Applied Scientific Research Center (BASRC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia

[3]Department of Immunology and Parasitology, Graduate School of Medicine University of the Ryukyus, Nishihara Cho, Japan

[4]Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

[5]Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

[6]National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

[7]National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

[8]College of Veterinary Medicine and Biomedical Sciences, Cavite State University, Indang, Cavite, Philippines

[9]National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

[10]Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt

[11]National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

[12]Livestock and Dairy Development Department, Veterinary Research Institute, Lahore, Punjab, Pakistan

[13]Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China

关键词: Babesia microti;    Plasmodium berghei;    co-infection;    cross immunity;    malaria;    babesiosis;    innate immunity;    acute stage immunity;   

DOI  :  10.3389/fcimb.2023.1226088

 received in 2023-05-20, accepted in 2023-06-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

Malaria remains one of the most significant health issues worldwide, accounting for 2.6% of the total global disease burden, and efforts to eliminate this threat continue. The key focus is to develop an efficient and long-term immunity to this disease via vaccination or therapeutic approach, and innovative strategies would enable us to achieve this target. Previously, using a mouse co-infection disease model, cross-protection was illustrated between Babesia microti and Plasmodium chabaudi. Hence, this study was planned to elucidate the impact of acute B. microti Peabody mjr and Plasmodium berghei ANKA co-infection on the consequence of complicated malaria in the C57BL/6J mouse model of malaria. Furthermore, immune response and pathological features were analyzed, and the course of the disease was compared among experimental groups. Our study established that acute B. microti infection activated immunity which was otherwise suppressed by P. berghei. The immunosuppressive tissue microenvironment was counteracted as evidenced by the enhanced immune cell population in co-infected mice, in contrast to P. berghei-infected control mice. Parasite sequestration in the brain, liver, lung, and spleen of co-infected mice was significantly decreased and tissue injury was ameliorated. Meanwhile, the serum levels of IFN-γ, TNF-α, and IL-12p70 were reduced while the secretion of IL-10 was promoted in co-infected mice. Eventually, co-infected mice showed an extended rate of survival. Hereby, the principal cytokines associated with the severity of malaria by P. berghei infection were TNF-α, IFN-γ, and IL-12p70. Moreover, it was evident from our flow cytometry results that innate immunity is crucial and macrophages are at the frontline of immunity against P. berghei infection. Our study recommended further investigations to shed light on the effects of babesiosis in suppressing malaria with the goal of developing Babesia-based therapy against malaria.

【 授权许可】

Unknown   
Copyright © 2023 Zafar, Taniguchi, Baghdadi, Kondoh, Rizk, Galon, Ji, El-Sayed, Do, Li, Amer, Zhuowei, Yihong, Zhou, Inoue and Xuan