Transcriptome analysis of two tobacco varieties with contrast resistance to Meloidogyne incognita in response to PVY MSNR infection

Frontiers in Plant Science

Plant Science

Pei Tian¹  Zhimin Jiang²  Bo Li²  Xiaoxiang Chen²  Jutao Sun³  Shixiao Xu³  Zhiqiang Zhang³ 

[1] China Tobacco Jiangsu Industry Co, Ltd. Xuzhou Cigarette Factory, Xuzhou, China;China Tobacco Zhejiang Industry Co, Ltd., Hangzhou, China;College of Tobacco Science, Henan Agricultural University, National Tobacco Cultivation & Physiology & Biochemistry Research Centre, Scientific Observation and Experiment Station of Henan, Ministry of Agriculture, Zhengzhou, China;

关键词: tobacco;    PVY MN;    root-knot nematode;    transcriptome;    resistance;   

DOI  :  10.3389/fpls.2023.1213494

 received in 2023-04-28, accepted in 2023-08-07,  发布年份 2023

来源: Frontiers

【 摘 要 】

Root-knot nematode (RKN) disease is a major disease of tobacco worldwide, which seriously hinders the improvement of tobacco yield and quality. Obvious veinal necrosis-hypersensitive responses are observed only in RKN-resistant lines infected by Potyvirus Y (PVY) MSNR, making this an effective approach to screen for RKN-resistant tobacco. RNA-seq analysis, real-time quantitative PCR (qRT-PCR) and functional enrichment analysis were conducted to gain insight into the transcription dynamics difference between G28 (RKN-resistant) and CBH (RKN-susceptible) varieties infected with PVY MSNR. Results showed that a total of 7900, 10576, 9921, 11530 and 12531 differentially expressed genes (DEGs) were identified between the two varieties at 0, 1, 3, 5, and 7 d after infection, respectively. DEGs were associated with plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and photosynthesis-related metabolic pathways. Additional DEGs related to starch and sucrose metabolism, energy production, and the indole-3-acetic acid signaling pathway were induced in CBH plants after infection. DEGs related to phenylpropanoid biosynthesis, abscisic acid, salicylic acid, brassinosteroids, and jasmonic acid signaling pathway were induced in G28 after infection. Our findings reveal DEGs that may contribute to differences in PVY MSNR resistance among tobacco varieties. These results help us to understand the differences in transcriptional dynamics and metabolic processes between RKN-resistant and RKN-susceptible varieties involved in tobacco-PVY MSNR interaction.

【 授权许可】

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Copyright © 2023 Xu, Tian, Jiang, Chen, Li, Sun and Zhang