Integrated analysis of transcriptome, metabolome, and histochemistry reveals the response mechanisms of different ages Panax notoginseng to root-knot nematode infection

Frontiers in Plant Science

Plant Science

Chen Ye¹  Yixiang Liu¹  Shusheng Zhu¹  Liwei Guo¹  Huiling Wang¹  Zhuhua Wang¹  Wentao Wu¹  Zhaoxia Wei¹  Youyong Zhu¹  Shuai Zhang¹  Hongping Huang¹  Yang Wang¹  Wenpeng Wang²  Xiahong He³ 

[1] State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, China;Academy of Science and Technology, Chuxiong Normal University, Chuxiong, Yunnan, China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, China;School of Landscape and Horticulture, Southwest Forestry University, Kunming, Yunnan, China;

关键词: Panax notoginseng;    root-knot nematode;    phenylpropanoid pathways;    lignin;    transcriptome;    metabolome;   

DOI  :  10.3389/fpls.2023.1258316

 received in 2023-07-13, accepted in 2023-08-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Panax notoginseng (P. notoginseng) is an invaluable perennial medicinal herb. However, the roots of P. notoginseng are frequently subjected to severe damage caused by root-knot nematode (RKN) infestation. Although we have observed that P. notoginseng possessed adult-plant resistance (APR) against RKN disease, the defense response mechanisms against RKN disease in different age groups of P. notoginseng remain unexplored. We aimed to elucidate the response mechanisms of P. notoginseng at different stages of development to RKN infection by employing transcriptome, metabolome, and histochemistry analyses. Our findings indicated that distinct age groups of P. notoginseng may activate the phenylpropanoid and flavonoid biosynthesis pathways in varying ways, leading to the synthesis of phenolics, flavonoids, lignin, and anthocyanin pigments as both the response and defense mechanism against RKN attacks. Specifically, one-year-old P. notoginseng exhibited resistance to RKN through the upregulation of 5-O-p-coumaroylquinic acid and key genes involved in monolignol biosynthesis, such as PAL, CCR, CYP73A, CYP98A, POD, and CAD. Moreover, two-year-old P. notoginseng enhanced the resistance by depleting chlorogenic acid and downregulating most genes associated with monolignol biosynthesis, while concurrently increasing cyanidin and ANR in flavonoid biosynthesis. Three-year-old P. notoginseng reinforced its resistance by significantly increasing five phenolic acids related to monolignol biosynthesis, namely p-coumaric acid, chlorogenic acid, 1-O-sinapoyl-D-glucose, coniferyl alcohol, and ferulic acid. Notably, P. notoginseng can establish a lignin barrier that restricted RKN to the infection site. In summary, P. notoginseng exhibited a potential ability to impede the further propagation of RKN through the accumulation or depletion of the compounds relevant to resistance within the phenylpropanoid and flavonoid pathways, as well as the induction of lignification in tissue cells.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Wang, Wu, Wang, Zhang, Ye, Guo, Wei, Huang, Liu, Zhu, Zhu, Wang and He