Frontiers in Cell and Developmental Biology | |
The bisphenol S contamination level observed in human follicular fluid affects the development of porcine oocytes | |
Cell and Developmental Biology | |
Jiří Moravec1  Jan Nevoral2  Young-Joo Yi3  Josef Fontana4  Jan Trnka4  Stanislava Martínková4  Kristýna Hošková5  Šárka Prokešová5  Jaroslav Petr5  Tereza Žalmanová5  Michal Benc6  Beáta Močáryová7  Michal Ješeta8  Jana Žáková8  Moustafa Elkalaf9  | |
[1] Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia;Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia;Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia;Department of Agricultural Education, College of Education, Sunchon National University, Suncheon, Republic of Korea;Department of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czechia;Centre for Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University, Prague, Czechia;Department of Biology of Reproduction, Institute of Animal Science, Prague, Czechia;Department of Biology of Reproduction, Institute of Animal Science, Prague, Czechia;Faculty of Natural Sciences and Informatics, Constantine the Philosopher University of Nitra, Nitra, Slovakia;Department of Biology of Reproduction, Institute of Animal Science, Prague, Czechia;Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czechia;Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, Brno, Czechia;Department of Pathophysiology, Third Faculty of Medicine, Charles University, Prague, Czechia; | |
关键词: bisphenol S (BPS); oocyte; aneuploidy; meiosis; follicular fluid (FF); endocrine disruption; embryonic development; | |
DOI : 10.3389/fcell.2023.1145182 | |
received in 2023-01-15, accepted in 2023-03-28, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information is available on the effects of Bisphenol S on ovarian follicles. In our study, we demonstrated the presence of Bisphenol S in the follicular fluid of women at a concentration of 22.4 nM. The effect of such concentrations of Bisphenol S on oocyte maturation and subsequent embryo development is still unknown. Therefore, we focused on the effect of Bisphenol S on in vitro oocyte maturation, fertilization, and embryo development. As a model, we used porcine oocytes, which show many physiological similarities to human oocytes. Oocytes were exposed to Bisphenol S concentrations similar to those detected in female patients in the ART clinic. We found a decreased ability of oocytes to successfully complete meiotic maturation. Mature oocytes showed an increased frequency of meiotic spindle abnormalities and chromosome misalignment. Alarming associations of oocyte Bisphenol S exposure with the occurrence of aneuploidy and changes in the distribution of mitochondria and mitochondrial proteins were demonstrated for the first time. However, the number and quality of blastocysts derived from oocytes that successfully completed meiotic maturation under the influence of Bisphenol S was not affected.
【 授权许可】
Unknown
Copyright © 2023 Žalmanová, Hošková, Prokešová, Nevoral, Ješeta, Benc, Yi, Moravec, Močáryová, Martínková, Fontana, Elkalaf, Trnka, Žáková and Petr.
【 预 览 】
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