The bisphenol S contamination level observed in human follicular fluid affects the development of porcine oocytes

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Jiří Moravec¹  Jan Nevoral²  Young-Joo Yi³  Josef Fontana⁴  Jan Trnka⁴  Stanislava Martínková⁴  Kristýna Hošková⁵  Šárka Prokešová⁵  Jaroslav Petr⁵  Tereza Žalmanová⁵  Michal Benc⁶  Beáta Močáryová⁷  Michal Ješeta⁸  Jana Žáková⁸  Moustafa Elkalaf⁹ 

[1] Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia;Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia;Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czechia;Department of Agricultural Education, College of Education, Sunchon National University, Suncheon, Republic of Korea;Department of Biochemistry, Cell and Molecular Biology, Third Faculty of Medicine, Charles University, Prague, Czechia;Centre for Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University, Prague, Czechia;Department of Biology of Reproduction, Institute of Animal Science, Prague, Czechia;Department of Biology of Reproduction, Institute of Animal Science, Prague, Czechia;Faculty of Natural Sciences and Informatics, Constantine the Philosopher University of Nitra, Nitra, Slovakia;Department of Biology of Reproduction, Institute of Animal Science, Prague, Czechia;Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czechia;Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, Brno, Czechia;Department of Pathophysiology, Third Faculty of Medicine, Charles University, Prague, Czechia;

关键词: bisphenol S (BPS);    oocyte;    aneuploidy;    meiosis;    follicular fluid (FF);    endocrine disruption;    embryonic development;   

DOI  :  10.3389/fcell.2023.1145182

 received in 2023-01-15, accepted in 2023-03-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information is available on the effects of Bisphenol S on ovarian follicles. In our study, we demonstrated the presence of Bisphenol S in the follicular fluid of women at a concentration of 22.4 nM. The effect of such concentrations of Bisphenol S on oocyte maturation and subsequent embryo development is still unknown. Therefore, we focused on the effect of Bisphenol S on in vitro oocyte maturation, fertilization, and embryo development. As a model, we used porcine oocytes, which show many physiological similarities to human oocytes. Oocytes were exposed to Bisphenol S concentrations similar to those detected in female patients in the ART clinic. We found a decreased ability of oocytes to successfully complete meiotic maturation. Mature oocytes showed an increased frequency of meiotic spindle abnormalities and chromosome misalignment. Alarming associations of oocyte Bisphenol S exposure with the occurrence of aneuploidy and changes in the distribution of mitochondria and mitochondrial proteins were demonstrated for the first time. However, the number and quality of blastocysts derived from oocytes that successfully completed meiotic maturation under the influence of Bisphenol S was not affected.

【 授权许可】

Unknown   
Copyright © 2023 Žalmanová, Hošková, Prokešová, Nevoral, Ješeta, Benc, Yi, Moravec, Močáryová, Martínková, Fontana, Elkalaf, Trnka, Žáková and Petr.