Chromosome cohesion decreases in human eggs with advanced maternal age

Aging Cell

Francesca E. Duncan²  Jessica E. Hornick²  Michael A. Lampson³  Richard M. Schultz³  Lonnie D. Shea¹ 

[1] Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA;Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA;Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA

关键词: aneuploidy;    cohesion;    egg;    human;    maternal age;    meiosis;   

DOI  :  10.1111/j.1474-9726.2012.00866.x

来源: Wiley

【 摘 要 】

Summary

Aneuploidy in human eggs increases with maternal age and can result in infertility, miscarriages, and birth defects. The molecular mechanisms leading to aneuploidy, however, are largely unknown especially in the human where eggs are exceedingly rare and precious. We obtained human eggs from subjects ranging from 16.4 to 49.7 years old following in vitro maturation of oocyte-cumulus complexes isolated directly from surgically removed ovarian tissue. A subset of these eggs was used to investigate how age-associated aneuploidy occurs in the human. The inter-kinetochore distance between sister chromatids increased significantly with maternal age, indicating weakened cohesion. Moreover, we observed unpaired sister chromatids from females of advanced age. We conclude that loss of cohesion with increasing maternal age likely contributes to the well-documented increased incidence of aneuploidy.

【 授权许可】

Unknown   
© 2012 The Authors. Aging Cell © 2012 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland