Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses

Frontiers in Physiology

Physiology

Klara Goethals¹  Guilherme Hosotani²  Jean Eduardo de Oliveira²  Luc Leybaert³  Constance de Meeûs d’Argenteuil⁴  Lorie de Maré⁴  Diete Lemmens⁴  Cathérine Delesalle⁴  Carmen Vidal Moreno de Vega⁴  Berit Boshuizen⁵  Lindsey Devisscher⁶  Filip Van Nieuwerburgh⁷  Dieter Deforce⁷ 

[1] Biometrics Research Center, Ghent University, Ghent, Belgium;Cargill R&D Centre Europe, Vilvoorde, Belgium;Department of Basic and Applied Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium;Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium;Wolvega Equine Hospital, Oldeholtpade, Netherlands;Gut-Liver Immunopharmacology Unit, Department of Basic and Applied Medical Sciences, Liver Research Center Ghent, Ghent University, Ghent, Belgium;Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium;

关键词: equine;    exercise;    training;    metabolism;    glucose;    glycogen;    energy;    fuel;   

DOI  :  10.3389/fphys.2023.1256217

 received in 2023-07-10, accepted in 2023-07-27,  发布年份 2023

来源: Frontiers

【 摘 要 】

Important changes in glucose transporter (GLUT) expression should be expected if the glucose influx plays a pivotal role in fuelling or connecting metabolic pathways that are upregulated in response to exercise. The aim was to assess GLUT4, 8, and 12 dynamics in response to training and acute exercise.Methods: Sixteen untrained Standardbred mares (3-4 year) performed an incremental SET at the start and end of 8 weeks harness training. M. pectoralis (PM) and M. vastus lateralis (VL) muscle biopsies were taken before and after each SET, allowing for comparing rest and acute samples in untrained (UT) and trained (T) condition using Western Blot for GLUT quantification and Image Pro v.10 for Blot analysis. Data were normalized against GAPDH. Basal GLUT-levels of PM versus VL were analysed with the Wilcoxon matched-pairs signed rank test. The effect of acute exercise or training was assessed using the Friedman test with a post hoc Dunn’s.Results: Basal GLUT4 and GLUT12 protein expression were significantly higher in the VL compared to the PM (PGLUT4 = 0.031 and PGLUT12 = 0.002). Training had no effect on basal GLUT4 expression, neither in the VL (p > 0.9999), nor the PM (p > 0.9999). However, acute exercise in trained condition significantly decreased GLUT4 expression in the VL (p = 0.0148). Neither training nor acute exercise significantly changed total GLUT8 protein expression. Training significantly decreased total GLUT12 protein expression in rest biopsies, only visible in the VL (p = 0.0359). This decrease was even more prominent in the VL after acute exercise in trained condition (PVL = 0.0025).Conclusion: The important changes seen in GLUT12 expression downregulation, both in response to training and acute exercise in the horse, the downregulation of GLUT4 expression after acute exercise in trained condition and the lack of differential shifts in GLUT8 expression in any of the studied conditions, questions the importance of glucose as substrate to fuel training and exercise in healthy horses. These findings encourage to further explore alternative fuels for their involvement in equine muscular energetics.

【 授权许可】

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Copyright © 2023 Vidal Moreno de Vega, Lemmens, de Meeûs d’Argenteuil, Boshuizen, de Maré, Leybaert, Goethals, de Oliveira, Hosotani, Deforce, Van Nieuwerburgh, Devisscher and Delesalle.