Modulation of the NO-cGMP pathway has no effect on olfactory responses in the Drosophila antenna

Frontiers in Cellular Neuroscience

Cellular Neuroscience

Bill S. Hansson¹  Sabine Kaltofen¹  Sinisa Prelic¹  Dieter Wicher¹  Merid N. Getahun² 

[1] Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, Jena, Germany;International Centre of Insect Physiology and Ecology, Nairobi, Kenya;

关键词: nitric oxide;    calcium imaging;    single sensillum recording;    insect olfaction;    olfactory sensory neuron;    cGMP;    cAMP;    cyclic nucleotide;   

DOI  :  10.3389/fncel.2023.1180798

 received in 2023-03-06, accepted in 2023-05-02,  发布年份 2023

来源: Frontiers

【 摘 要 】

Olfaction is a crucial sensory modality in insects and is underpinned by odor-sensitive sensory neurons expressing odorant receptors that function in the dendrites as odorant-gated ion channels. Along with expression, trafficking, and receptor complexing, the regulation of odorant receptor function is paramount to ensure the extraordinary sensory abilities of insects. However, the full extent of regulation of sensory neuron activity remains to be elucidated. For instance, our understanding of the intracellular effectors that mediate signaling pathways within antennal cells is incomplete within the context of olfaction in vivo. Here, with the use of optical and electrophysiological techniques in live antennal tissue, we investigate whether nitric oxide signaling occurs in the sensory periphery of Drosophila. To answer this, we first query antennal transcriptomic datasets to demonstrate the presence of nitric oxide signaling machinery in antennal tissue. Next, by applying various modulators of the NO-cGMP pathway in open antennal preparations, we show that olfactory responses are unaffected by a wide panel of NO-cGMP pathway inhibitors and activators over short and long timescales. We further examine the action of cAMP and cGMP, cyclic nucleotides previously linked to olfactory processes as intracellular potentiators of receptor functioning, and find that both long-term and short-term applications or microinjections of cGMP have no effect on olfactory responses in vivo as measured by calcium imaging and single sensillum recording. The absence of the effect of cGMP is shown in contrast to cAMP, which elicits increased responses when perfused shortly before olfactory responses in OSNs. Taken together, the apparent absence of nitric oxide signaling in olfactory neurons indicates that this gaseous messenger may play no role as a regulator of olfactory transduction in insects, though may play other physiological roles at the sensory periphery of the antenna.

【 授权许可】

Unknown   
Copyright © 2023 Prelic, Getahun, Kaltofen, Hansson and Wicher.