Frontiers in Immunology | |
Identification of immune activation-related gene signature for predicting prognosis and immunotherapy efficacy in lung adenocarcinoma | |
Immunology | |
Jin Wang1  Baisong Li1  Mi Liu1  Qifan Li2  Hao Ding2  Jun Chen2  Zhike Chen2  Yuan Cui2  Sheng Ju2  Jian Yang2  Gaomeng Luo2  Jun Zhao2  Yufeng Xie2  Xin Tong2  Weibiao Zeng2  | |
[1] Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou, China;Institute of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China;Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China; | |
关键词: lung adenocarcinoma; immune activation; immune infiltration; immunotherapy efficacy; prognosis; | |
DOI : 10.3389/fimmu.2023.1217590 | |
received in 2023-05-05, accepted in 2023-06-23, 发布年份 2023 | |
来源: Frontiers | |
![]() |
【 摘 要 】
BackgroundLung adenocarcinoma (LUAD) is a major subtype of non-small cell lung cancer (NSCLC) with a highly heterogeneous tumor microenvironment. Immune checkpoint inhibitors (ICIs) are more effective in tumors with a pre-activated immune status. However, the potential of the immune activation-associated gene (IAG) signature for prognosis prediction and immunotherapy response assessment in LUAD has not been established. Therefore, it is critical to explore such gene signatures.MethodsRNA sequencing profiles and corresponding clinical parameters of LUAD were extracted from the TCGA and GEO databases. Unsupervised consistency clustering analysis based on immune activation-related genes was performed on the enrolled samples. Subsequently, prognostic models based on genes associated with prognosis were built using the last absolute shrinkage and selection operator (LASSO) method and univariate Cox regression. The expression levels of four immune activation related gene index (IARGI) related genes were validated in 12 pairs of LUAD tumor and normal tissue samples using qPCR. Using the ESTIMATE, TIMER, and ssGSEA algorithms, immune cell infiltration analysis was carried out for different groups, and the tumor immune dysfunction and rejection (TIDE) score was used to evaluate the effectiveness of immunotherapy.ResultsBased on the expression patterns of IAGs, the TCGA LUAD cohort was classified into two clusters, with those in the IAG-high pattern demonstrating significantly better survival outcomes and immune cell infiltration compared to those in the IAG-low pattern. Then, we developed an IARGI model that effectively stratified patients into different risk groups, revealing differences in prognosis, mutation profiles, and immune cell infiltration within the tumor microenvironment between the high and low-risk groups. Notably, significant disparities in TIDE score between the two groups suggest that the low-risk group may exhibit better responses to ICIs therapy. The IARGI risk model was validated across multiple datasets and demonstrated exceptional performance in predicting overall survival in LUAD, and an IARGI-integrated nomogram was established as a quantitative tool for clinical practice.ConclusionThe IARGI can serve as valuable biomarkers for evaluating the tumor microenvironment and predicting the prognosis of LUAD patients. Furthermore, these genes probably provide valuable guidance for establishing effective immunotherapy regimens for LUAD patients.
【 授权许可】
Unknown
Copyright © 2023 Zeng, Wang, Yang, Chen, Cui, Li, Luo, Ding, Ju, Li, Chen, Xie, Tong, Liu and Zhao
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202310104308808ZK.pdf | 7912KB | ![]() |