Identifying Immune Cell Infiltration and Effective Diagnostic Biomarkers in Lung Adenocarcinoma by Comprehensive Bioinformatics Analysis and In Vitro Study

Frontiers in Oncology

RuiQing Zhai¹  William C. Cho²  Xi Chen³  Xinyan Li⁴  Yixiao Yuan⁵  Xiulin Jiang⁷ 

[1] College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China;Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, Hong Kong SAR, China;Department of Neurosurgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China;Department of Pharmacy, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China;Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, China;Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, China;Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China;

关键词: FAM72B;    lung adenocarcinoma;    prognosis biomarker;    immune infiltration;    ceRNA;   

DOI  :  10.3389/fonc.2022.916947

来源: DOAJ

【 摘 要 】

Family with sequence similarity 72B (FAM72B) has been characterized in the regulation of neuronal development. Nevertheless, the prognostic value of FAM72B expression and its function in the immune microenvironment of lung adenocarcinoma (LUAD) currently remains elusive. In this study, by adopting bioinformatics methodology and experimental verification, we found that FAM72B was upregulated in lung cancer tissues and cell lines, and a higher FAM72B level predicted an unfavorable clinical outcome in LUAD patients. The knockdown of FAM72B significantly inhibited cell proliferation, cell migration, and induced cell apoptosis in LUAD. The receiver operating characteristic curve suggested that FAM72B had a high predictive accuracy for the outcomes of LUAD. Kyoto Encyclopedia of Genes and Genomes and Gene Set Enrichment Analyses confirmed that FAM72B-related genes were involved in cell proliferation and immune-response signaling pathway. Moreover, upregulated FAM72B expression was significantly associated with immune cell infiltration in the LUAD tumor microenvironment. Meanwhile, a potential ceRNA network was constructed to identify the lncRNA-AL360270.2/TMPO-AS1/AC125807.2/has-let-7a/7b/7c/7e/7f/FAM72B regulatory axis that regulates FAM72B overexpression in LUAD and is associated with a poor prognosis. We also confirmed that AL360270.2, TMPO-AS1, and AC125807.2 were significantly upregulated in LUAD cell lines than in human bronchial epithelial cells. In conclusion, FAM72B may serve as a novel biomarker in predicting the clinical prognosis and immune status for lung adenocarcinoma.

【 授权许可】

Unknown