Long Non-Coding RNA AL139385.1 as a Novel Prognostic Biomarker in Lung Adenocarcinoma

Frontiers in Oncology

Wenjun Ren¹  Xi Chen²  Jun Pu²  Xiaobin Huang²  Xiaoqun Niu³  Fan Zhou⁴  Jishu Guo⁵  Xiulin Jiang⁶ 

[1] Department of Cardiovascular Surgery, The First People’s Hospital of Yunnan Province, Kunming, China;Department of Neurosurgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China;Department of Respiratory Medicine, Second Hospital of Kunming Medical University, Kunming, China;Hematology and Rheumatology Department, The Pu’er People’s Hospital, Pu’er, China;Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming, China;Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China;

关键词: AL139385.1;    lung adenocarcinoma;    prognosis biomarker;    DNA methylation;    ceRNA;    cell proliferation;   

DOI  :  10.3389/fonc.2022.905871

来源: DOAJ

【 摘 要 】

Lung adenocarcinoma (LUAD) is the most common histological lung cancer, and it is the leading cause of cancer-related deaths worldwide. LncRNA-AL139385.1 (ENSG00000275880) is a novel lncRNA that is abnormally expressed in various cancer types including LUAD. However, the underlying biological function and potential mechanisms of AL139385.1 driving the progression of LUAD remain unclear. In this study, we investigated the role of AL139385.1 in LUAD and found that DNA hypomethylation was positively correlated with AL139385.1 expression in LUAD. Moreover, we uncover that the expression of AL139385.1 in LUAD tissues was significantly higher than that of AL139385.1 expression in adjacent normal tissues. Kaplan–Meier survival analysis showed that patients with higher AL139385.1 expression correlated with adverse overall survival and progression-free survival. Receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) value of AL139385.1 was 0.808. Correlation analysis showed that AL139385.1 expression was associated with immune infiltration in LUAD. We also found that AL139385.1 was upregulated in LUAD cancer tissues and cell lines. Knockdown of AL139385.1 significantly inhibited cell proliferation and migration abilities of LUAD. Finally, we constructed a ceRNA network that includes hsa-miR-532-5p and four mRNAs (GALNT3, CYCS, EIF5A, and ITGB4) specific to AL139385.1 in LUAD. Subsequent Kaplan–Meier survival analysis suggested that polypeptide N-acetylgalactosaminyltransferase 3 (GALNT3), cytochrome c, somatic (CYCS), eukaryotic translation initiation factor 5A (EIF5A), and integrin subunit beta 4 (ITGB4), were potential prognostic biomarkers for patients with LUAD. In conclusion, this finding provides possible mechanisms underlying the abnormal upregulation of AL139385.1 as well as a comprehensive view of the AL139385.1-mediated competing endogenous RNAs (ceRNA) network in LUAD, thereby highlighting its potential role in diagnosis and therapy.

【 授权许可】

Unknown