Adipose tissue-derived exosomes contribute to obesity-associated liver diseases in long-term high-fat diet-fed mice, but not in short-term

Frontiers in Nutrition

Nutrition

Andre Kim¹  Taesang Son²  Inae Jeong²  Ok-Kyung Kim³  Jeongjin Park³  Woojin Jun³ 

[1] Department of Pharmaceutical Engineering, Silla University, Busan, Republic of Korea;Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea;Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea;Human Ecology Research Institute, Chonnam National University, Gwangju, Republic of Korea;

关键词: exosome;    obesity;    insulin resistance;    high-fat diet;    lipogenesis;   

DOI  :  10.3389/fnut.2023.1162992

 received in 2023-02-10, accepted in 2023-04-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionOur study aimed to investigate the changes in hepatic endoplasmic reticulum (ER) stress, inflammation, insulin signaling, and lipid metabolism during the administration of a high-fat diet (HFD) in mice in order to identify correlations between obesity and metabolic disease development in the liver.MethodsWe used short-, medium-, and long-term HFD periods, corresponding to 4, 8, and 12 weeks, respectively, and isolated exosomes from adipose tissue. We confirmed the effect of adipose tissue-derived exosomes on metabolic disorders in obesity in alpha mouse liver 12 (AML12) hepatocytes.ResultsAdipose tissue-derived exosomes from HFD mice did not affect the AML12 cells after 4 weeks, but ER stress, inflammatory response, insulin resistance, and lipid synthesis were observed after 8 and 12 weeks. Furthermore, we confirmed that an HFD increases the amount of adipose tissue-derived exosomes in mice. Consequently, we can infer that adipose tissue-derived exosomes from HFD-fed mice significantly increase ER stress, inflammatory response, insulin resistance, and lipid synthesis in AML12 cells.DiscussionOur results demonstrate that obesity alters the effects of adipose tissue-derived exosomes in the liver, potentially becoming a risk factor in the development of obesity-induced liver diseases.

【 授权许可】

Unknown   
Copyright © 2023 Son, Jeong, Park, Jun, Kim and Kim.