Isolation of a diazinon-degrading strain Sphingobium sp. DI-6 and its novel biodegradation pathway

Frontiers in Microbiology

Microbiology

Guangli Wang¹  Xuedong Li¹  Xiang Li¹  Jiaxin Zheng¹  Lingling Bai¹  Wenlong Yue²  Li Jiang³ 

[1] Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, School of Life Sciences, Huaibei Normal University, Huaibei, China;Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, China;State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, China;

关键词: Sphingobium;    diazinon-biodegradation metabolites;    novel metabolic pathway;    16S rRNA;    environmental remediation;   

DOI  :  10.3389/fmicb.2022.929147

 received in 2022-04-26, accepted in 2022-07-26,  发布年份 2022

来源: Frontiers

【 摘 要 】

Diazinon is one of the most widely used organophosphate insecticides, one that is frequently detected in the environment. In this study, a diazinon-degrading bacterium, DI-6, previously isolated from diazinon-contaminated soil in China has been subsequently identified as Sphingobium sp. on the basis of its physiological and biochemical characteristics, as well as by virtue of a comparative analysis of 16S rRNA gene sequences. This strain is capable of using diazinon as its sole carbon source for growth and was able to degrade 91.8% of 100 mg L–1 diazinon over a 60-h interval. During the degradation of diazinon, the following seven metabolites were captured and identified by gas chromatography/mass spectrometry (GC–MS) analysis: diazoxon, diazinon aldehyde, isopropenyl derivative of diazinon, hydroxyethyl derivative of diazinon, diazinon methyl ketone, O-[2-(1-hydroxyethyl)-6-methylpyrimidin-4-yl] O-methyl O-hydrogen phosphorothioate, and O-(6-methyl pyrimidin-4-yl) O,O-dihydrogen phosphorothioate. Based on these metabolites, a novel microbial biodegradation pathway of diazinon by Sphingobium sp. DI-6 is proposed. This research provides potentially useful information for the application of the DI-6 strain in bioremediation of diazinon-contaminated environments.

【 授权许可】

Unknown   
Copyright © 2022 Wang, Li, Zheng, Li, Bai, Yue and Jiang.