Enriching drought resistance in Solanum lycopersicum using Abscisic acid as drought enhancer derived from Lygodium japonicum: A new-fangled computational approach

Frontiers in Plant Science

Plant Science

Najat A. Bukhari¹  Aisha Saleh Alwadai¹  Kahkashan Perveen¹  Najla A. Alshaikh¹  Alanoud T. Alfagham¹  Sandip Debnath²  Dong-Qing Wei³ 

[1]Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

[2]Department of Genetics and Plant Breeding, Institute of Agriculture, Visva-Bharati University, Sriniketan, West Bengal, India

[3]Tianjin Institute of Bioinformatics and Drug Discovery (TIBDD), Tianjin Normal University, Tianjin, China

关键词: phytochemicals;    drought;    MD simulation;    molecular docking;    drought tolerant;    Solanum lycopersicum;    Abscisic acid;   

DOI  :  10.3389/fpls.2023.1106857

 received in 2022-11-24, accepted in 2023-01-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionDrought is the largest abiotic factor impacting agriculture. Plants are challenged by both natural and artificial stressors because they are immobile. To produce drought-resistant plants, we need to know how plants react to drought. A largescale proteome study of leaf and root tissue found drought-responsive proteins. Tomato as a vegetable is grown worldwide. Agricultural biotechnology focuses on creating drought-resistant cultivars. This is important because tomato drought is so widespread. Breeders have worked to improve tomato quality, production, and stress resistance. Conventional breeding approaches have only increased drought tolerance because of drought’s complexity. Many studies have examined how tomatoes handle drought. With genomics, transcriptomics, proteomics, metabolomics, and modern sequencing technologies, it’s easier to find drought-responsive genes.MethodBiotechnology and in silico studies has helped demonstrate the function of drought-sensitive genes and generate drought-resistant plant types. The latest tomato genome editing technology is another. WRKY genes are plant transcription factors. They help plants grow and respond to both natural and artificial stimuli. To make plants that can handle stress, we need to know how WRKY-proteins, which are transcription factors, work with other proteins and ligands in plant cells by molecular docking and modeling study.ResultAbscisic acid, a plant hormone generated in stressed roots, was used here to make plants drought-resistant. Abscisic acid binds WRKY with binding affinity -7.4kcal/mol and inhibitory concentration (Ki) 0.12 microM.DiscussionThis study aims to modulate the transcription factor so plants can handle drought and stress better. Therefore, polyphenols found to make Solanum lycopersicum more drought-tolerant.

【 授权许可】

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Copyright © 2023 Perveen, Alfagham, Debnath, Bukhari, Wei, Alshaikh and Alwadai