Tumor-targeted bioactive nanoprobes visualizing of hydrogen peroxide for forecasting chemotherapy-exacerbated malignant prognosis

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Fangman Chen¹  Li Li²  Fan Zhang²  Zhimin Chang²  Yong Jia³  Yawei Zhao³ 

[1] CAS Key Laboratory of Bio Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences, Suzhou, China;CAS Key Laboratory of Bio Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences, Suzhou, China;Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China;School of Nursing, Jilin University, Changchun, Jilin, China;

关键词: bioactive;    nanoprobe;    cancer;    hydrogen peroxide;    chemotherapy;   

DOI  :  10.3389/fbioe.2023.1226680

 received in 2023-05-22, accepted in 2023-07-17,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Fluorescent visualization of hydrogen peroxide in the tumor microenvironment (TME) is conducive to predicting malignant prognosis after chemotherapy. Two photon microscopy has been employed for in vivo hydrogen peroxide detection owing to its advantages of deep penetration and low phototoxicity.Methods: In this study, a two-photon fluorescent probe (TPFP) was protected by mesoporous silica nanoparticles (MSNs) and masked by cloaking the cancer cell membranes (CM), forming a tumor-targeted bioactive nanoprobe, termed MSN@[e-mail]: This multifunctional nanoprobe allowed for the effective and selective detection of excessive hydrogen peroxide production in chemotherapeutic Etoposide (VP-16)-challenged tumor cells using two-photon microscopy. After specific accumulation in tumors, VP-16-MSN@TPFP@CM monitored tumor-specific hydrogen peroxide levels and revealed a positive correlation between oxidative stress in the TME and chemotherapy-exacerbated malignant prognosis.Discussion: Given the recent translation of fluorescent imaging into early clinical trials and the high biocompatibility of bioactive nanoprobes, our approach may pave the way for specific imaging of oxidative stress in solid tumors after treatment and provide a promising technology for malignant prognosis predictions.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Jia, Chen, Zhao, Li and Chang.