Isolation, characterization, and pathogenicity assessment of Corynebacterium pseudotuberculosis biovar equi strains from alpacas (Vicugna pacos) in China

Frontiers in Microbiology

Microbiology

Tingmei He¹  Wanyu Meng²  Haifeng Liu²  Jinpeng Yang²  Lin Huang²  Ziyao Zhou²  Hualin Fu²  Zhijun Zhong²  Guangneng Peng²  Shanyu Chen²  Wenqing Zhang² 

[1] Sichuan Wolong National Natural Reserve Administration, Wenchuan, Sichuan, China;The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China;

关键词: alpaca;    Corynebacterium pseudotuberculosis;    biovar equi;    virulence;    antibiotic resistance;    pathogenicity;    genomic sequencing;   

DOI  :  10.3389/fmicb.2023.1206187

 received in 2023-04-15, accepted in 2023-06-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

Corynebacterium pseudotuberculosis is a zoonotic pathogen that causes lymphadenitis in humans, livestock, and wildlife. In this study, C. pseudotuberculosis biovar equi strains were isolated from three alpacas. Antibiotic susceptibility tests and pathogenicity tests were also conducted. Moreover, one strain was sequenced using DNBSEQ and Oxford Nanopore technology. The three strains exhibited resistance to aztreonam, fosfomycin, and nitrofurantoin. The median lethal doses (LD50) of strains G1, S2 and BA3 in experimentally infected mice was 1.66 × 105 CFU, 3.78 × 105 CFU and 3.78 × 105 CFU, respectively. The sequencing of strain G1 resulted in the assembly of a chromosomal scaffold comprising 2,379,166 bp with a G + C content of 52.06%. Genome analysis of strain G1 revealed the presence of 48 virulence genes and 5 antibiotic resistance genes (ARGs). Comparative genomic analysis demonstrates a high degree of genetic similarity among C. pseudotuberculosis strains, in contrast to other Corynebacterium species, with a clear delineation between strains belonging to the two biovars (ovis and equi). The data of the present study contribute to a better understanding of the properties of C. pseudotuberculosis biovar equi strains and the potential risk they pose to alpacas and other livestock, as well as the necessity of ongoing surveillance and monitoring of infectious diseases in animals.

【 授权许可】

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Copyright © 2023 Meng, Chen, Huang, Yang, Zhang, Zhong, Zhou, Liu, Fu, He and Peng.