Stereotaxic cutting of post-mortem human brains for neuroanatomical studies

Frontiers in Neuroanatomy

Neuroanatomy

Carmen Cavada¹  Isabel Pérez-Santos¹  Miguel Ángel García-Cabezas² 

[1] Department of Anatomy, Histology and Neuroscience, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain;Ph.D. Program in Neuroscience Cajal-UAM, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain;Department of Anatomy, Histology and Neuroscience, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain;Ph.D. Program in Neuroscience Cajal-UAM, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain;Neural Systems Laboratory, Department of Health Sciences, Boston University, Boston, MA, United States;

关键词: stereotaxis;    stereotaxic instrument;    Talairach;    human neuroanatomy;    intercommissural plane;   

DOI  :  10.3389/fnana.2023.1176351

 received in 2023-02-28, accepted in 2023-04-24,  发布年份 2023

来源: Frontiers

【 摘 要 】

Stereotaxis is widely used in clinical neurosurgery, neuroradiosurgery, and neuroimaging. Yet, maps of brain structures obtained from post-mortem human brains are not usually presented in known stereotaxic coordinates. Post-mortem brain data given in stereotaxic coordinates would facilitate comparisons with in vivo human neuroimages and would also facilitate intra and inter-experiment comparisons. In this article, we present a crafted instrument for stereotaxic cutting of post-mortem human brain hemispheres. The instrument consists of a transparent methacrylate plate facing a mirror, four legs, and lateral regularly spaced columns permitting the insertion of large knives in-between the columns. This instrument can be built in any laboratory to obtain human brain slabs in the stereotaxic space of Talairach and Tournoux. We explain in detail the procedure for stereotaxic cutting of human brain hemispheres in the coronal plane, as well as the basis for calculating stereotaxic coordinates of histological sections obtained following the stereotaxic cutting protocol.

【 授权许可】

Unknown   
Copyright © 2023 García-Cabezas, Pérez-Santos and Cavada.