Influence of intra-abomasal administration of L-DOPA on circulating catecholamines and feed intake in cattle

Frontiers in Animal Science

Animal Science

Eriton E. L. Valente¹  Gustavo W. Costa¹  Amanda M. Egert-McLean²  David L. Harmon³  John B. May⁴  James L. Klotz⁵ 

[1] Animal Science Department, Western Paraná State University, Marechal Cândido Rondon, PR, Brazil;Department of Animal Science, University of Tennessee, Knoxville, TN, United States;Department of Animal and Food Science, University of Kentucky, Lexington, KY, United States;Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, United States;Forage-Animal Production Research Unit, USDA-ARS, Lexington, KY, United States;

关键词: dopamine;    levodopa;    metabolism;    neurotransmitter;    serotonin;   

DOI  :  10.3389/fanim.2023.1127575

 received in 2022-12-19, accepted in 2023-01-31,  发布年份 2023

来源: Frontiers

【 摘 要 】

Dopamine has multiple physiological functions including feed intake control in which it can act as an anorectic or orexigenic agent. This study had the objective to evaluate intra-abomasal administration of L-DOPA (levodopa; L-3,4-dihydroxyphenylalanine) from -Mucuna pruriens on circulating catecholamines, indicators of energy metabolism and feed intake in cattle. Eight Holstein steers (340 ± 20 kg) fitted with ruminal cannula were used in a replicated 4 x 4 Latin Square design experiment. Intra-abomasal infusion of L-DOPA at 0, 0.5, 1 and 2 mg/kg BW was carried out for seven days and blood samples were collected at 0, 30, 60, 120, 240 and 480 min from L-DOPA infusion on day 7. The area under the curve (AUC) of plasma L-DOPA and free dopamine increased quadratically with the administration of L-DOPA. However, the AUC of plasma total dopamine had a positive linear response with the increase of L-DOPA. Conversely, the serum 5-hydroxytriptophan (5-HTP), plasma serotonin, serum serotonin, serum tyrosine, plasma glucose and plasma free fatty acids were not affected by the intra-abomasal infusion of L-DOPA. The circulating concentration of the epinephrine, norepinephrine, serotonin, glucose and free fatty acids did not change with L-DOPA infusion. It can be concluded that intra-abomasal L-DOPA administration produced a strong increase in circulating dopamine with no change in energy metabolites and feed intake in cattle.

【 授权许可】

Unknown   
Copyright © 2023 Valente, Klotz, Egert-McLean, Costa, May and Harmon