The altered metabolites contributed by dysbiosis of gut microbiota are associated with microbial translocation and immune activation during HIV infection

Frontiers in Immunology

Immunology

Ningmei Liu¹  Yanjun Li¹  Sufang Ai¹  Zhiman Xie¹  Qisi Su¹  Chuanyi Ning²  Ping Cui³  Peijiang Pan³  Jingzhen Lai³  Yanyan Liao³  Jie Zhou⁴  Jiegang Huang⁴  Zhuoxin Li⁴  Li Ye⁴  Hao Liang⁵  Yu Zhang⁶  Qijian Su⁷ 

[1] Department of Infectious Diseases, The Fourth People's Hospital of Nanning, Nanning, China;Guangxi Key Laboratory of AIDS Prevention and Treatment and Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, Nanning, China;Guangxi Key Laboratory of AIDS Prevention and Treatment and Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, Nanning, China;Life Science Institute, Guangxi Medical University, Nanning, China;Guangxi Key Laboratory of AIDS Prevention and Treatment and Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, Nanning, China;School of Public Health, Guangxi Medical University, Nanning, China;Guangxi Key Laboratory of AIDS Prevention and Treatment and Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, Nanning, China;School of Public Health, Guangxi Medical University, Nanning, China;Life Science Institute, Guangxi Medical University, Nanning, China;Guangxi Key Laboratory of AIDS Prevention and Treatment and Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, Nanning, China;School of Public Health, Guangxi Medical University, Nanning, China;The Tenth Affiliated Hospital of Guangxi Medical University, Qinzhou, China;The Tenth Affiliated Hospital of Guangxi Medical University, Qinzhou, China;

关键词: HIV;    gut microbiota;    metabolism;    immune activation;    microbial translocation;   

DOI  :  10.3389/fimmu.2022.1020822

 received in 2022-08-16, accepted in 2022-11-28,  发布年份 2023

来源: Frontiers

【 摘 要 】

BackgroundThe immune activation caused by microbial translocation has been considered to be a major driver of HIV infection progression. The dysbiosis of gut microbiota has been demonstrated in HIV infection, but the interplay between gut microbiota and its metabolites in the pathogenesis of HIV is seldom reported.MethodsWe conducted a case-controlled study including 41 AIDS patients, 39 pre-AIDS patients and 34 healthy controls. Both AIDS group and pre-AIDS group were divided according to clinical manifestations and CD4 + T cell count. We collected stool samples for 16S rDNA sequencing and untargeted metabolomics analysis, and examined immune activation and microbial translocation for blood samples.ResultsThe pre-AIDS and AIDS groups had higher levels of microbial translocation and immune activation. There were significant differences in gut microbiota and metabolites at different stages of HIV infection. Higher abundances of pathogenic bacteria or opportunistic pathogen, as well as lower abundances of butyrate-producing bacteria and bacteria with anti-inflammatory potential were associated with HIV severity. The metabolism of tryptophan was disordered after HIV infection. Lower level of anti-inflammatory metabolites and phosphonoacetate, and higher level of phenylethylamine and polyamines were observed in HIV infection. And microbial metabolic pathways related to altered metabolites differed. Moreover, disrupted metabolites contributed by altered microbiota were found to be correlated to microbial translocation and immune activation.ConclusionsMetabolites caused by dysbiosis of gut microbiota and related metabolic function are correlated to immune activation and microbial translocation, suggesting that the effect of microbiota on metabolites is related to intestinal barrier disruption in HIV infection.

【 授权许可】

Unknown   
Copyright © 2023 Zhang, Xie, Zhou, Li, Ning, Su, Ye, Ai, Lai, Pan, Liu, Liao, Su, Li, Liang, Cui and Huang