Environmental factors and host genotype control foliar epiphytic microbial community of wild soybeans across China

Frontiers in Microbiology

Microbiology

Pablo García-Palacios¹  Manuel Delgado-Baquerizo²  Guang Yang³  Hui-Ling Cui⁴  Ming Yan⁴  Gui-Lan Duan⁴  Yue Yin⁴  Xing-Yun Yi⁴  Rui Zhou⁴  Lv Li⁴  Yong-Guan Zhu⁵ 

[1] Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Madrid, Spain;Laboratorio de Biodiversidad y Funcionamiento Ecosistémico, Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), CSIC, Sevilla, Spain;Unidad Asociada CSIC-UPO (BioFun), Universidad Pablo de Olavide, Sevilla, Spain;State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China;University of Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China;University of Chinese Academy of Sciences, Beijing, China;Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China;

关键词: wild soybean;    foliar microbiome;    microbial community assembly;    core microbiome;    host genotype;   

DOI  :  10.3389/fmicb.2023.1065302

 received in 2022-10-09, accepted in 2023-02-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionThe microbiome inhabiting plant leaves is critical for plant health and productivity. Wild soybean (Glycine soja), which originated in China, is the progenitor of cultivated soybean (Glycine max). So far, the community structure and assembly mechanism of phyllosphere microbial community on G. soja were poorly understood.MethodsHere, we combined a national-scale survey with high-throughput sequencing and microsatellite data to evaluate the contribution of host genotype vs. climate in explaining the foliar microbiome of G. soja, and the core foliar microbiota of G. soja were identified.ResultsOur findings revealed that both the host genotype and environmental factors (i.e., geographic location and climatic conditions) were important factors regulating foliar community assembly of G. soja. Host genotypes explained 0.4% and 3.6% variations of the foliar bacterial and fungal community composition, respectively, while environmental factors explained 25.8% and 19.9% variations, respectively. We further identified a core microbiome thriving on the foliage of all G. soja populations, including bacterial (dominated by Methylobacterium-Methylorubrum, Pantoea, Quadrisphaera, Pseudomonas, and Sphingomonas) and fungal (dominated by Cladosporium, Alternaria, and Penicillium) taxa.ConclusionOur study revealed the significant role of host genetic distance as a driver of the foliar microbiome of the wild progenitor of soya, as well as the effects of climatic changes on foliar microbiomes. These findings would increase our knowledge of assembly mechanisms in the phyllosphere of wild soybeans and suggest the potential to manage the phyllosphere of soya plantations by plant breeding and selecting specific genotypes under climate change.

【 授权许可】

Unknown   
Copyright © 2023 Zhou, Duan, García-Palacios, Yang, Cui, Yan, Yin, Yi, Li, Delgado-Baquerizo and Zhu.