Metabolomic Variability of Different Soybean Genotypes: β-Carotene-Enhanced (Glycine max), Wild (Glycine soja), and Hybrid (Glycine max × Glycine soja) Soybeans

Foods

Sang-Un Park¹  Sun-Hwa Ha²  Jae-Kwang Kim³  Jung-Won Jung³  Soo-Yun Park⁴  Yejin Jang⁴  Sung-Dug Oh⁴  Sang-Jae Suh⁵  Soon-Ki Park⁵ 

[1] Department of Crop Science and Department of Smart Agriculture Systems, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea;Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea;Division of Life Sciences, Incheon National University, Incheon 22012, Korea;National Institute of Agricultural Sciences, Rural Development Administration (RDA), Jeonju-si 55365, Korea;School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea;

关键词: metabolic profiling;    metabolite analysis;    hybrid soybean;    β-carotene-enhanced soybean;    wild soybean;   

DOI  :  10.3390/foods10102421

来源: DOAJ

【 摘 要 】

We obtained a new hybrid soybean (Hybrid) by hybridizing β-carotene-enhanced soybean (BCE; Glycine max L.) containing the phytoene synthase-2A-carotene desaturase gene and wild-type soybean (Wild; Glycine soja). To investigate metabolic changes between variants, we performed metabolic profiling of leaves (three growth stages) and seeds. Multivariate analyses revealed significant metabolic differences between genotypes in seeds and leaves, with seeds showing accumulation of phytosterols, tocopherols, and carotenoids (BCE only), indicating co-induction of the methylerythritol 4-phosphate and mevalonic acid pathways. Additionally, Hybrid produced intermediate levels of carotenoids and high levels of amino acids. Principal component analysis revealed metabolic discrimination between growth stages of soybean leaves and identified differences in leaf groups according to different genotypes at 8, 12, and 16 weeks, with Wild showing higher levels of environmental stress-related compounds relative to BCE and Hybrid leaves. The metabolic profiling approach could be a useful tool to identify metabolic links in various soybean cultivars.

【 授权许可】

Unknown